High Fidelity Taq DNA Polymerase

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Product Overview

Description


Product Description

High Fidelity Taq DNA Polymerase

Taq Plus DNA Polymerase is a kind of High Fidelity Taq DNA Polymerase.mixture of Taq DNA Polymerase and proofreading DNA Polymerase,which allows for the amplification of long templates, up to 30kb, with high fidelity. The two enzymes act synergistically during PCR to generate more accurate and longer PCR products with greater yields compared to Taq DNA Polymerase alone. PCR products, amplified up to 10kb in length with Taq Plus DNA Polymerase, generate a mixture of blunt ends and single base (A) 3' overhang. The products can be used for direct T/A cloning,but its efficiency is not as high as PCR products amplified with Taq polymerase alone.

Concentration: 5u/µl
Store at –20°C

Description

Taq Plus DNA Polymerase is a special formulation designed for amplify large fragment. The main component is Taq DNA Polymerase, and Pfu DNA Polymerase are added to enhance the efficiency of amplification reaction. Theoretically, Taq Plus produces significantly higher yields of PCR products than ordinary Taq Polymerase, especially for fragments >1kb, and can amplify up to 20kb. Taq Plus also contains a proofreading activity that reduces the error rate of Taq Polymerase. Most of the amplified DNA fragments have a 3´A overhanging. However, a small percentage of the amplified DNA fragments are blunt-ended. Taq Plus is suitable as a direct replacement for ordinary Taq Polymerase in most applications.

Features

High fidelity: with an error frequency of 1.6X10-6 during DNA synthesis.
Higher yield: Taq Plus increases the efficiency of polymerization reaction, resulting in a great percentage of extenuation reaction completion up to 20kb

Unit Definition:
One unit of the enzyme catalyzes the incorporation of 10nmole of deoxyribonucleotides into a polynucleotide fraction in 30min at 74℃

Quality Control
No contaminating endonuclease or exonuclease activity detected. Functionally tested in PCR.

Storage

20mM Tris-HCl, pH 8.0, 100mM KCl, 0.1mM EDTA, 5mM DTT, 50% glycerol, 0.5% NP40 and 0.5% Tween 20.

10X Reaction Buffer (Mg2+ Plus):
500mM KCl, 100mM Tris-HCl (pH 9.0 at 25℃) and 1% Triton X-100, 100mM (NH4)2SO4, 15mM MgCl2, PCR enhancer

Application

Long PCR (up to 20 kb), PCR cloning, RT-PCR etc.

Aftersale service

.ISO 9001:2000 certifi ed Quality Management System
International Authorized Third-Party Test For The Products You Demand.
Free lot-specifi c qualifi cation samples
Individual delivery schedules
Custom testing and documentation
Custom formulations, packaging & labeling
Free lot-specifi c qualifi cation samples
Service
1.Fast respond ,any inquiries will be replied within 12 hours.
2. Strong commitment and dedication to quality and service.
3. Customer requirment is the commercial information for both of us, we will kept strictly confidential
4. OEM/ODM Available.
5. Reasonable & competitive price, fast lead time.

Our Company

Tinzyme is a professional molecular bio reagent Manufacture in China.The key products are nucleic acid substances and the enzymes/ Polymerase for molecular biology. We have many patents for the the nuceic acid and the enzymes. And the monomers of the nucleic are all enzymatically synthesized using our proprietary unique technology.These dNTPs and NTPs are functionally approved for all PCR- and related reactions including Real Time PCR, in vitro transcription assays and DNA-sequencing in diagnostics and molecular biology. Also the enzymes such as Taq DNA polymerase, M-MLV Reverse Transcriptase, pfu DNA polymerase are all worked very well. The perfomrace of our products can compared to the top bio companys of the world.


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