Plasmid DNA extraction Kit Spin Column form

Product Overview

Description

Easy Spain plasmid miniprep kit

 

Component	Storage	50 Preps  (PL0301)	100 Preps  (PL0302)
Buffer P1 *	4 °C	15 ml	25 ml
Buffer P2	RT	15 ml	25 ml
Buffer P3	RT	20 ml	35 ml
Buffer PE*	RT	16 ml                  31.5 ml Add indicated ethanol before first use
Buffer WB*	RT	15 ml                  25 ml Add indicated ethanol before first use
Elution Buffer (EB)	RT	15 ml	15 ml
RNase A (10mg / ml)	–20 °C	150 μl	250 μl
DNA Binding Columns	RT	50	100

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Introduction:

The Miniprep system provides a fast, simple, and cost-effective plasmid miniprep method for routine molecular biology laboratory applications. Miniprep Kits use a modified alkaline lysis method and silica membrane technology to eliminate the cumbersome steps associated with loose resins or slurries. Phenol extraction and ethanol precipitation are not required. Plasmid DNA purified with Miniprep Kits is immediately ready for use. The plasmid DNA is suitable for a variety of experiments in molecular biology, including automated fluorescent DNA sequencing, restriction enzyme digestion, or transformation.

 

Features:

1.Organic extractions or ethanol precipitation is not required.

2.Up to 20 μg high-purity plasmid or cosmid in 30 minutes or less.

3.DNA is suitable for routine downstream applications.

 

 

Store :RNase A: store at -20°C.

Before starting, please add RNase A to Buffer PI, mix well and store at 4°C.

Other reagent can be store at room temperature.

 

If precipitate forms in the buffers during storage, it should be redissolved by incubating the buffers at 37°C before use.

Packaging size:

 

50 preps/ box  18boxes per carton
100 preps/ box  18boxes per carton