Feed grade thermostable and acid-resistant phytase enzyme for animal additive Nutrizyme PHY
INTRODUCTION
Phytase ultra (Phytase Premier I) [EC 3.1.3.26] is produced from Aspergillus niger through advanced fermentation and extraction technology.
It is thermostable and good tolerant to pepsin and trypsin. It can break down the undigestible phytic acid (phytate) and release digestible phosphorus, calcium and other nutrients. As an animal feed additive used in poultry and swine, it can enhance the nutritive value by liberation of inorganic phosphate from phytic acid (myo-inositol hexakisphosphate) , thereby, reduce phosphorus pollution to the environment.
DEFINITION OF UNIT
1 unit of phytase equals to the amount of enzyme, which liberates 1 μmol of inorganic phosphorus in 1 min, from 5.0mmol/L sodium phytase at 37℃ and pH5.0.
SPECIFICATIONS
ENZYME ACTIVITY
5,000u/g;10000u/g
APPEARANCE
White or Yellowish powder
Products can be customized according to customer 's demand.
DOSAGE
Used for Complete feed
Growth stage
Dosage: (g/MT)
≥5000 u/g
≥10,000 u/g
swine
20-35kg
150
75
More than 35kg
120
60
poultry
Whole period
200
100
layer
Brooding period
200
100
Laying hens
60
30
Laying duck
80
40
ENZYMES PROPOTIES
1.Enzyme properties at different pH conditions
The optimum pH of Sunson Phytase Premier I was 3.5 and the pH range was from 2.5 to 5.5. Compared to the current mainstream phytase in international market, Sunson phytase Premier I showed higher activity between pH2.5 to 5.5, which was more suitable for the digestion of phytic acid in animal gastrointestinal tract.
Fig. 1.Relation between phytase activity and pH
Note:The Mainstream phytase means the current mainstream phytase in international market.
2. Temperature stability
The activity of Phytase Premier I could maintain 80% or more, which results showed the phytase had a very good heat resistance.
(water bath for 3min under 75℃~95℃) Fig. 2.Relation between phytase activity and temperature
3. Stability under the feed pelleting process
An experiment was conducted in a feed factory in Shandong province. The phytase was fixed with the swine's feed, conditioned for 90s at 85℃,0.4MPa and pelleted for 10s in a Modulator (AG,CH-9240, Bühler Co., Wuxi China), testing the thermostability during feed pelleting process.
Source
Phytase Premier I
Mainstream phytase
retention rate
84%
88%
Phytase Premier I maintained high activity under feed pelleting process.
4.Tolerance to endogenous protease
Table 2 Resistance to pepsin and trypsin
Condition
Resistance to pepsin
Resistance to trypsin
buffer
buffer+ pepsin
buffer
buffer+ trypsin
pH
2.5
2.5
6.5
6.5
Temperature
40℃
40℃
40℃
40℃
Time
1.5h
1.5h
2h
2h
Protease concentration
__
1mg/ml
__
2.5mg/ml
Compared to the current mainstream phytase in international market, Sunson phytase Premier I has a better resistance to endogenous protease.
5. Phosphorus release capacity
The phytase was mixed with soybean meal, incubated at 40℃ for 4h at pH 3.0, then transfered to pH 6.5, 40℃ for 4h. Phosphorus release was determined as below.
Results showed Phytase Premier I had much higher phosphorus release capacity than the mainstream phytase.
FUNCTIONS & BENEFITS
1. Effectively hydrolyze phytic acid in the plant materials, increase the phosphorus utilization rate of feed raw material; 2. Reduce the dosage of inorganic phosphorus and save cost of animal feed. 3. Break phytanate frame, release nutrients, like: starch, protein, mineral elements, increase the utilization rate; 4. Improve the absorption phosphorus and reduce environmental pollution.
ADVANTAGES
1. Produced through advanced liquid fermentation and extraction technology; 2. Good resistance to endogenic protease and gastric acid; 3. Thermostable, wide pH range, catalyzing effectively in in animal gastrointestinal tract. 4. Even granular, appropriate grain size, blend equally; 5. Highly concentrated and cost saving.
PACKAGE&STORAGE
1. Packaging specification: 25 KGS/Bag. 2.The shelf life is 12 months under 20℃. 3. Damp and insolation should be avoided. Store it in a cool, dry and ventilated place.
ABOUT US
Company introduction
Sunson Industry Group Co., Ltd. was founded in 1996. After 21 years full efforts, Sunson has grown to be a large enzyme enterprise, specializing in exploring, researching, manufacturing and marketing of industrial enzymes. We have 1 strain R & D center, 1 application research center and 2 modern manufacturing bases. Sunson group is the member of China Biotech Fermentation Industry Association, and has been awarded as "Key Enterprise of China Enzymes Industry". We take the lead to be certified with ISO9001:2000 quality management system in enzyme industry.
Currently, Sunson group has established 6 subsidiary companies and 30 offices in 32 cities, provinces and municipalities in China. Our products have been exported to more than 30 countries and regions. "Sunson" has become a leading brand of enzyme industry in China.
OUR SERVICE
1. Any inquiries will be replied within 12 hours. 2. Dedication to quality, supply & service. 3. Strictly on selecting raw materials. 4. OEM/ODM Available. 5. Reasonable & competitive price, fast lead time. 6. Sample is available for your evaluation & Formulation development. 7. Faster delivery: Sample order in stock and 3-7 days for bulk production. 8. We have strong cooperation with DHL, TNT, UPS, FEDEX, EMS. Or you also can choose your own shipping forwarder.
