Genomic DNA Extraction Kit DNA Purification Kit Spin column form

Product Overview

Description

Tissue Blood Genomic DNA Extraction Kit DNA Purification Kit

 

Description

Uses the silica-gel-membrane technology  isolate  Genomic DNA without phenol/chloroform.

Homogenization is not necessary,  directly lysed by Proteinase K.

Optimized to allow selective binding of DNA to the silica-gel membrane.

The simple centrifugation protocol completely removes contaminants such as proteins, divalent cations, and secondary metabolites.

Pure DNA is then eluted in water or low-salt buffer, ready to use.

Suitable for anticoagulated blood. 400 μl whole blood yield 3-10 μg high pure genomic DNA. The purified DNA can be used in common downstream applications such as sequencing, restriction digestion,etc.

 

Feature.

 

High Efficient – 3-10 ug genomic DNA from 400 ul whole blood

Safe - no phenol/cholroform extraction step.

High purity - Purified DNA is ready for downstream application such as PCR, restriction digestion.

 

Applications

Purified DNA is free from contaminants and enzyme inhibitors, and typically has A₂₆₀/A₂₈₀ ratios between 1.7 and 1.9, and is suitable for applications such as:

 

Restriction digestion

PCR

Labeling

Southern hybridisation

Library construction

 

 

 

Store

store Protein K at -20°C