high sensitivity easy Taq DNA Polymerase in bulk 10U/ul of High Fidelity
- Category: Specific Reagents >>>
- Supplier: Shenzhen Tinzyme Gene Tech Co. Limited
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Product Overview
Description
Product Description10 U/μl
10 U/μl
Isolated from the E. coli cloning Thermus aquaticus.
The molecular weight is approximately 94 kDa.
EasyTaq DNA polymerase has the 5′to 3′DNA polymerase activity and 5′to 3′exonuclease activity without 3′-5′exonuclease activity.
The extending speed is 1-2 kb/min.There is an "A" on 3′end.The PCR product can be cloned in TA vector.
Conc. 10 U/μl
Characteristics:
high-sensitivity
high amplification efficiency
Unit Definition:
One unit of Platinum Taq DNA Polymerase High Fidelity incorporates 10 nmol of deoxyribonucleotide into acid-preceptible material in 30 minutes at 74°C.
Storage Buffer: 20 mM Tris-HCl (pH 8.0),0.1 mM EDTA,1 mM DTT,100 mM KCl,Stabilizers,50% glycerol.
10X PCR reaction Buffer: 500 mM KCl,100 mM Tris-Cl (pH8.5 at 25℃),1% Triton X-100,15 mM MgCl2
Reaction Mixture Set Up
Component Volume Final Concentration
Template DNA <0.5 ug as required
Forward Primer (10 μM) 1 μl 0.2-0.4 μM each
Reverse Primer (10 μM) 1 μl 0.2-0.4 μM each
10×PCR reaction Buffer 5 μl 1×
2.5 mM dNTPs 4 μl 0.2 mM
Taq DNA polymerase 0.5μl 2.5 unit
ddH2O to final volume 50μl Not applicable
Recommended thermal cycling conditions
94℃ 2-5 min 30-35 cycles
94℃ 30 sec 30-35 cycles
50-60℃ 30 sec 30-35 cycles
72℃ 1-2 kb/min 30-35 cycles
72℃ 5-10 min 30-35 cycles
Detailed Images
