SYBR Green I is a very sensitive dsDNA detection dye of 10000x for qPCR
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- Supplier: Shenzhen Tinzyme Gene Tech Co. Limited
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Product Overview
Description
Product Description
SYBR Green I nucleic acid gel stain was developed specifically for reduced mutagenicity—to
be safer than ethidium bromide for staining DNA in agarose or acrylamide gels. SYBR gel stain
is not only less mutagenic than ethidium bromide, but SYBR gel stain’s detection sensitivity is
better than that of ethidium bromide. SYBR gel stain comes as a premixed solution that can be
used just like an ethidium bromide solution, either in the gel during the electrophoresis run or
as a post-run stain. Bound to nucleic acids, the green-fluorescent SYBR gel stain has
fluorescence excitation maxima at ~280 and ~502 nm, and an emission maximum at ~530 nm
The dye is supplied as a 10,000x solution in dimethyl sulfoxide (DMSO). One ml of solution
prepares 10 liters of Staining Solution, sufficient for 100 mini gels
Protocal
Gel soaking
Classical method for agarose and polyacrylamide gels.
1.Run sample(s) in agarose or polyacrylamide gel.
2.In a beaker, add 10 uL of 10,000x SYBR Green I solution in DMSO to 100 mL of 1x TE, TBE,
or TAE buffer (for mini gel), or 50 uL of 10,000x SYBR Green I solution in DMSO to 500 mL of
1x TE, TBE, or TAE buffer (for mid-sized gel). Mix thoroughly with spatula, rod, or magnetic
stirrer.
3.Pour the diluted SYBR Green I solution in appropriate tray or pan.
4.Soak the gel for 5-10 min.
5.View or document the gel using 254 nm low-pressure mercury lamp and orange filter.
Gel pre-staining
This method is acceptable for agarose gels only, but not for PAAG.
1.Boil the agarose in buffer to dissolution using microwave or heating appliance.
2.While hot, add 1 uL of 10,000x SYBR Green I solution in DMSO per each 10 mL of gel
solution. Mix thoroughly.
3.Pour the gel and let it cool down.
For best results, add 1 uL of of 10,000x SYBR Green I solution in DMSO per each 10 mL of
buffer near anode ("+", red wire).
4.Run the samples. Real-time monitoring of migrating bands under 254 nm low-pressure
mercury lamp possible.
5.View or document the gel using 254 nm low-pressure mercury lamp and orange filter
Classical method for agarose and polyacrylamide gels.
1.Run sample(s) in agarose or polyacrylamide gel.
2.In a beaker, add 10 uL of 10,000x SYBR Green I solution in DMSO to 100 mL of 1x TE, TBE,
or TAE buffer (for mini gel), or 50 uL of 10,000x SYBR Green I solution in DMSO to 500 mL of
1x TE, TBE, or TAE buffer (for mid-sized gel). Mix thoroughly with spatula, rod, or magnetic
stirrer.
3.Pour the diluted SYBR Green I solution in appropriate tray or pan.
4.Soak the gel for 5-10 min.
5.View or document the gel using 254 nm low-pressure mercury lamp and orange filter.
Gel pre-staining
This method is acceptable for agarose gels only, but not for PAAG.
1.Boil the agarose in buffer to dissolution using microwave or heating appliance.
2.While hot, add 1 uL of 10,000x SYBR Green I solution in DMSO per each 10 mL of gel
solution. Mix thoroughly.
3.Pour the gel and let it cool down.
For best results, add 1 uL of of 10,000x SYBR Green I solution in DMSO per each 10 mL of
buffer near anode ("+", red wire).
4.Run the samples. Real-time monitoring of migrating bands under 254 nm low-pressure
mercury lamp possible.
5.View or document the gel using 254 nm low-pressure mercury lamp and orange filter
Detailed Images
Packing & Delivery
Delivery:
Faster delivery:Sample order in stock, and 3-7 working days for bulk orders after payment,shiping with blue ice
Storage:
Long Term (Infrequent use; 1-2 times per month): -70℃
Daily/Weekly use: -20℃
Related Products
We also have oligo, hot star taq, proteinase K and PCR and so on! If you need more ,Please do not hesitate to contact us.
Skype:mebep.tech
Email:sales at tinzyme dot com
Mob/Whatsapp/Viber:+86 13760337582
FAQ
Title goes here.
Q: Are you a manufacturer?
A: Yes, we are factory founded on 1988.
A: Yes, we are factory founded on 1988.
Q: Do you offer free samples?
A: Yes we offer free samples for most of our products, such as dNTPs, Taq DNA Polymerase, HotSTar Taq DNA Polymerase, M-MLV and so on. While the shipping charge the customer side.
Q:What's the payment method of you company?
We only accept the payment by T/T, Western Union or Money Gram. If the order amount lower than 150 USD we can accept by PayPal.
Q:Do you provide warranties for your products?
A:Yes we offer one years warrantee from the purchsed day.
Q:Do you accept the small orders?
A:Yes, we accept the small orders. We will try our best to support our customer's business whether the small trail order
Q:How many Freeze-thaw cycles will not affect the activity of the dNTPs?
A: 20 Freeze-thaw cycles do not affect the dNTPs activity. Even thoughwe suggest avoid freeze-thaw cycles or exposure to frequent temperature changes.
Q: How Many days the dNTPs can be stable in room temperature?
A: We have made this tes before, and or dNTPs can be stable in RT at least for 10 days.
After sale service
.ISO 9001:2000 certifi ed Quality Management System
International Authorized Third-Party Test For The Products You Demand.
Free lot-specifi c qualifi cation samples
Individual delivery schedules
Custom testing and documentation
Custom formulations, packaging & labeling
Free lot-specifi c qualifi cation samples
Service
1.Fast respond ,any inquiries will be replied within 12 hours.
2. Strong commitment and dedication to quality and service.
3. Customer requirment is the commercial information for both of us, we will kept strictly confidential
4. OEM/ODM Available.
5. Reasonable & competitive price, fast lead time.
Our Company
Title goes here.
Tinzyme is a professional molecular bio reagent Manufacture in China.The key products are nucleic acid substances and the enzymes/ Polymerase for molecular biology. We have many patents for the the nuceic acid and the enzymes. And the monomers of the nucleic are all enzymatically synthesized using our proprietary unique technology.These dNTPs and NTPs are functionally approved for all PCR- and related reactions including Real Time PCR, in vitro transcription assays and DNA-sequencing in diagnostics and molecular biology. Also the enzymes such as Taq DNA polymerase, M-MLV Reverse Transcriptase, pfu DNA polymerase are all worked very well. The perfomrace of our products can compared to the top bio companys of the world.
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