The Gold Start Taq DNA Polymerase is a recombinant Taq DNA polymerase which has been chemically modified by the addition of heat-labile blocking groups to its amino acid residues. The enzyme is inactive at room temperature, avoiding extension of non-specifically annealed primers or primer dimers and providing higher specificity of DNA amplification. The functional activity of the enzyme need to be activated in incubation at 95°C for 10 minute. The activated enzyme maintains the same functionality as Taq DNA polymerase: Has the 5′to 3′DNA polymerase activity and 5′to 3′exonuclease activity without 3′-5′exonuclease activity. The extending speed is 1 kb/30 sec. There is an “A” on 3′end.The PCR product can be cloned in TA vector.
Concentration: 5 u/μl
Applications: Hot-start PCR amplification Specific amplification of complex cDNA and genomic template Amplification from low copy number DNA template Real-Time PCR Multiple PCR Generation of PCR products for TA cloning, Gene microarray analysis and SNP test
Reaction
Component
Volume
Final Concentration
Template DNA
<1 ug
as required
Forward Primer (10 μM)
2 μl
0.4 μM
Reverse Primer (10 μM)
2 μl
0.4 μM
5×GoldStarTaq PCR Buffer
10 μl
1×
2.5mM each dNTPmix
4 μl
200μM each
GoldStar Taq DNA polymerase, 5U/μl
0.5μl
2.5 unit
ddH2O to final volume
50μl
Not applicable
Quality Control
Functional absence of double and single-stranded endonuclease activity; Purity>99% test by SDS gel electrophoresis; Each lot of GoldStar Taq DNA Polymerase is assayed for amplification from as little as 10 ng of human genomic DNA ; Retain full activity at room temperature for one week; No host DNA residue .
Packing & Delivery
Delivery:
Faster delivery:Sample order in stock, and 3-7 working days for bulk orders after payment,shiping with blue ice
Storage:
Store at -20°C
Package：in bulk
Related Products
MMLV, bst DNA polymerase, RNAse inhibitor, UNG thermal liable,hot star taq, proteinase K both avilable, If you need more ,Please do not hesitate to contact us.
Skype:mebep.tech
Email:sales at tinzyme dot com
Mob/Whatsapp/Viber:+86 18922896756
FAQ
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Q: Are you a manufacturer? A: Yes, we are factory founded on 1988.
Q: Do you offer free samples? A: Yes we offer free samples for most of our products, such as dNTPs, Taq DNA Polymerase, HotSTar Taq DNA Polymerase, M-MLV and so on. While the shipping charge the customer side.
Q:What's the payment method of you company? We only accept the payment by T/T, Western Union or Money Gram. If the order amount lower than 150 USD we can accept by PayPal.
Q:Do you provide warranties for your products? A:Yes we offer one years warrantee from the purchsed day.
Q:Do you accept the small orders? A:Yes, we accept the small orders. We will try our best to support our customer's business whether the small trail order
Q:How many Freeze-thaw cycles will not affect the activity of the dNTPs? A: 20 Freeze-thaw cycles do not affect the dNTPs activity. Even thoughwe suggest avoid freeze-thaw cycles or exposure to frequent temperature changes.
Q: How Many days the dNTPs can be stable in room temperature? A: We have made this tes before, and or dNTPs can be stable in RT at least for 10 days.
After sale service
.ISO 9001:2000 certifi ed Quality Management System
International Authorized Third-Party Test For The Products You Demand. Free lot-specifi c qualifi cation samples
1.Fast respond ,any inquiries will be replied within 12 hours. 2. Strong commitment and dedication to quality and service. 3. Customer requirment is the commercial information for both of us, we will kept strictly confidential 4. OEM/ODM Available. 5. Reasonable & competitive price, fast lead time.
Our Company
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Tinzyme is a professional molecular bio reagent Manufacture in China.The key products are nucleic acid substances and the enzymes/ Polymerase for molecular biology. We have many patents for the the nuceic acid and the enzymes. And the monomers of the nucleic are all enzymatically synthesized using our proprietary unique technology.These dNTPs and NTPs are functionally approved for all PCR- and related reactions including Real Time PCR, in vitro transcription assays and DNA-sequencing in diagnostics and molecular biology. Also the enzymes such as Taq DNA polymerase, M-MLV Reverse Transcriptase, pfu DNA polymerase are all worked very well. The perfomrace of our products can compared to the top bio companys of the world.
