Diagnostic Test device Trichinella Spiralis Ab ELISA Test Kit

Product Overview

Description

Trichinella Spiralis Antibody ELISA Kit

 

1.Usage

The Trichinella spiralis(TS)Ab ELISA is a Enzyme Linked Immunosorbent  Assay for the qualitative detection of specific  antibodies to Porcine trichinella spiralis in Swine serum.

 

2.Principle

This kit use indirect ELISA method,Pured TS antigen is pre-coated on enzyme micro-well strips. When testing, add diluted serum sample after incubation, it will combine with the pre-coated antigen if the TS virus is specific antibody . Discard the uncombined antibody and other components with washing .Then add enzyme label anti-TS virus monoclonal VP1 . Antibody in sample block the combination of monoclonal antibody and pre-coated antigen, Discard the uncombined enzyme conjugate with washing; Add TMB substrate in micro-wells, the blue signal by Enzyme catalysis is in inverse proportion of antibody content in sample, use ELISA reader at 450nm wavelenth to measure the absorbance A value in reaction wells after adding stop solution to stop the reaction.

 

3.The Kit components

  Reagent Volume                                                                   96 Tests/192Tests
  Antigen coated micro-assay plate                                               1ea/2ea
  Negative Control                                                                       0.8ml/1.6l
  Positive Control                                                                         0.8ml/1.6ml
  Sample dilute solution                                                                    50/100ml
  Washing solution (10X concentrated)                                            50/100ml
  Enzyme conjugate                                                                            6/11ml
  Color liquid                                                                                      11/22ml
  Stopping solution                                                                              6/11ml

  Adhesive plate sealer                                                                   2ea/4ea
  Dilution microplate                                                                         1ea/2ea
  Product specification                                                                       1 pcs

 

4.Produc  e of Elisa test

 

5. Interpretation of the Results
1) Test Validation
  a. The mean absorbance value of negative control(NCx) is < 0.2.
  b. The mean absorbance value of Positive control(PCx) is > 0.5
  c. If these values are out of range, result should be considered invalid and the samples should be retested.
2) Calculation of the Result
Criteria : The criteria is based on following formula.
IRPC = mean OD450 of sample- mean OD450 of negative /mean OD450 of Positive e control- mean OD450 of negative
3) Interpretation of Results
The status of samples is determined as follows;
- IRPC≥ 0.4 is considered positive.
- IRPC < 0.4 is considered negative.

 

 

 

Our Service

 

1. The inquiries will be replied quickly within 24 hours.

2. Professional manufacturer focus on Animal diagnostic test. Welcome to visit our factory.

3. OEM service are available.

   A. Customized design box.

   B. Uncut sheet is available.

   C. Label is available.

   D. Any your idea on diagnostic test we can help you to research and put it into production.

4. High quality, reasonable and competitive price

5. Good After-sale service

   A. All products will have been strictly quality checked in the warehouse before packing.

   B. All products will be well packed before shipping.

   C.Our products have 12 months expiration date.

6. Free samples

7. Delivery Time

  Sample order in stock and 7 days for bulk production

8. Payment

  You can pay for the order via: T/T, Western Union. L/C

9 Shipping

  We cooperate with DHL, Fedex, TNT

  You also can use your own shipping forwarder.

 

Copmpany Information

 

Shenzhen ZhenRui Biotechnology Co.,Ltd is a HIgh-tech enterprise. All of the products are manufactured strictly under

ISO9001 control system. We are focused on animal disease diagnosis.