Taq Plus DNA Polymerase for GC rich pcr P1031 P1032 P1033 P1034

Product Overview

Description

Taq Plus DNA Polymerase

#P1031, 250U

Concentration: 2.5U/μl

Contents:

Taq Plus DNA Polymerase	100 μl
10× PCR Buffer (Mg2+ Free)	1.25 ml
6× Loading Buffer	1 ml
25mM MgCl2	1.25 ml

 

Description
Taq Plus, a mixture of taq and pfu polymerase, blends the processivity of taq with the high fidelity of pfu. Therefore, this specially formulated Taq plus allow amplification of the higer fidelity and longer templates than the single-enzyme formulations. It is also a better choice for amplifying complex template, such as GC-rich template. And it is suitable as a direct replacement for ordinary Taq Polymerase in most applications. In addition, Using Taq plus results in 3´-dA overhangs PCR products, which can be used in TA clone.

Features
High fidelity: Its fidelity is four times higher than ordinary Taq polymerase.
High yields: Suitable for amplifying large fragment, suitable for complex template which is rich in GC or repeat sequence.

Applications

•  Amplification of long template up to 20kb

•  Amplification of complex template

•  High fidelity PCR

Quality Control
The absence of endodeoxyribonucleases, exodeoxyribonucleases and ribonucleases confirmed by appropriate quality tests

Functionally tested in PCR

Definition of Activity Unit
One unit is defined as the amount of the enzyme required to catalyze the incorporation of 10 nM of dNTPs into an acid-insoluble form in 30 minutes at 70°C using hering sperm DNA as substrate.

Storage Buffer
20 mM Tris-HCl, 1 mM DTT, 0.1 mM EDTA, 100 mM KCl, 0.5 % TW 20, 0.5 % NP 40, 50 % Glycerol

10X PCR Buffer with Mg²⁺
500 mM KCl, 100 mM Tris-Cl, 15 mM MgCl2, 1% Triton X-100

 

Store all components at –20°C

 

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Dongsheng Biotech PCR Reagents are looking for distributors and providing OEM service

Dongsheng Biotech offers different series of products to help you achieve PCR success. For enzymes, our Taq Polymerase, HS Taq DNA Polymerase, FS Taq DNA Polymerase, Pfu DNA Polymerase and Fusion Pfu DNA Polymerase provide high fidelity, efficient, sensitivity PCR performance. We also have Long Taq DNA Polymerase for long PCR performance.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Dongsheng Biotech Co., Ltd takes PCR technology as its core, and its products focus on common PCR, qPCR, nucleic acid detection and other fields. Adhering to the quality policy of "unremitting pursuit of continuous innovation, leading technology and customer satisfaction", we provide our customers with cost-effective molecular biology products.