Бензо эндонуклеаза, бензо нуклеаза

Product Overview

Description

Benzo Nuclease Manufactuered under GMP contitions

 

 

Benzo Nuclease is a kinds of no-specific endonuclease. The Benzo genetically engineered endonuclease from Serratia marcescens.

Benzo Nuclease degrades all kinds of DNA and RNA (double stranded,single stranded, linear and circular and supercoil) but without proteolytic activity. It is effective over a wide range of conditions and possesses an exceptionally high specific activity.

Benzo completely digests nucleic acids to 5'monophosphate terminated oligonucleotides 2 to 5 bases in length (below the hybridization limit), which is ideal for removal of nucleic acids from recombinant proteins, enabling compliance with FDA guidelines for nucleic acid contamination.

It is functional between pH 6 and 10 and from 0-42°C and requires 1-2mM Mg2+for activation. The enzyme is also active in the presence of ionic and non-ionic detergents, reducing agents, PMSF (1 mM), EDTA (1 mM) and urea (relative activity depends on specific conditions). Activity is inhibited by > 150 mM monovalent cations, > 100 mM phosphate, > 100 mM ammonium sulfate, or > 100 mM guanidine HCl.

 

Color:Clear

 

Activity:>= 250U/μl

 

Specific activity>=1.0x10⁶U/mg protien

 

Protease: Not detectable

 

Microbial limit: aerobe<10CFU/100 KU Yeast and mold:<10CFU/ 100 KU

 

Endotoxin Test:(Gel Clot LAL Assay) <0.25EU/1000U

 

Shipping and Handling:

 

Ship Blue Ice Only Standard Handling

 

Guarantee:2 years

 

Applification:

 

1. removal of nucleic acids from recombinant proteins, enabling compliance with FDA guidelines for nucleic acid contamination.

2. rapidly hydrolyze nucleic acids makes the enzyme an excellent choice for viscosity reduction to reduce processing time and increase yields of protein.

3. For particles in the process to improve the purification process of particles Nucleic acid is easy to stick in virus particles, the surface of inclusion particle cells such as particle, cause these The particle size of particles or charge change and influence the separation. Nuclease can effectively avoid the nucleic acid Influence on the purification and increase production

4.Used for biochemical analysis in the preparation of the sample In ELISA, chromatographic analysis, bipolar electrophoresis and footprints in the analysis, processing with nucleic acid enzymes containing the nucleic acid samples Is tasted, can improve the resolution and improve recovery.