Veterinary equipment Brucellosis antibody ELISA Test Bovine sheep brucella Elisa Kit

Product Overview

Description

Veterinary equipment Brucellosis antibody  ELISA Test Bovine sheep brucella Elisa Kit

 

 

1. Principle of the Test

The Brucellosis antibody ELISA kit is used to test Brucellosis antibody in serum of bovine, sheep, pig and dog etc.

This kit use competitive ELISA method, Brucellosis antigen is pre-coated on enzyme micro-well strips. When testing, add diluted serum sample, after incubation, if there is Brucellosis specific antibody, it will combine with the pre-coated antigen, discard the uncombined antibody and other components with washing; then add enzyme labled anti-Brucellosis virus monoclonal antibody, antibody in sample block the combination of monoclonal antibody and pre-coated antigen; discard the uncombined enzyme conjugate with washing; Add TMB substrate in micro-wells, the blue signal by Enzyme catalysis is in inverse proportion of antibody content in sample, use ELISA reader at 450nm wavelenth to measure the absorbance A value in reaction wells after adding stop solution to stop the reaction

2. Materials Provided
	Reagent	Volume
		96 Tests/192Tests
1	Antigen  coated micro-assay plate	1ea/2ea
2	Negative Control	1.5ml/2.5ml
3	Positive Control	0.5ml/1ml
4	Sample diluents	6/12ml
5	Washing solution (10X concentrated)	100ml
6	Enzyme conjugate	6/12ml
8	Stopping solution	6/11ml
9	Adhesive plate sealer	2ea/4ea
10	Color liquid	11/22ml
11	Instruction	1 pcs

 

 

 

  Interpretation of the Results

 

Set zero at blank control well, read the OD value at 450nm (630nm as reference).

For the assay to be valid:

OD value of negative control(N) > 0.5, meanwhile positive value (P) blocking rate > 80%

Calculate method:

PI(blocking rate)= {1- (Sample OD value/ Negative control OD average value)}x 100%

Results interpretation

PI(blocking rate)> 70%: Positive

PI(blocking rate)≤ 70%: Negative

 

 

 

   

FMDV Type O Ab rapid test card	FMDV Type O Ab Elisa Kit
FMDV Ag rapid test card	FMDV Type A Ab ELISA Kit
FMDV Type A Ab rapid test card	Cystic echinococcosis ab Elisa kit
Peste des petits ruminants virus    Antibody rapid test kit card	Peste des petits ruminants virus    Antibody Elisa Kit
Brucellosis Antibody rapid test card	Brucellosis Ab ELISA Kit
Brucellosis Antigen rapid test card	Bovine Tuberculosis Ab Elisa kit
Rotavirus Ag rapid test kit card	FMDV Type AsiaⅠAb ELISA Kit
Bovine Tuberculosis Ab rapid test	FMDV NSP 3abc Elisa kit