Veterinary equipment Bovine Brucellosis Ab Elisa Test Kit Quantitative Elisa Kit

Product Overview

Description

Veterinary equipment Bovine Brucellosis Ab Elisa Test Kit

 

 

1. Principle of the Test

The Brucellosis antibody ELISA kit is used to test Brucellosis antibody in serum of bovine, sheep, pig and dog etc.This kit use competitive ELISA method, Brucellosis antigen is pre-coated on enzyme micro-well strips. When testing, add diluted serum sample, after incubation, if there is Brucellosis specific antibody, it will combine with the pre-coated antigen, discard the uncombined antibody and other components with washing; then add enzyme labled anti-Brucellosis virus monoclonal antibody, antibody in sample block the combination of monoclonal antibody and pre-coated antigen; discard the uncombined enzyme conjugate with washing; Add TMB substrate in micro-wells, the blue signal by Enzyme catalysis is in inverse proportion of antibody content in sample, use ELISA reader at 450nm wavelenth to measure the absorbance A value in reaction wells after adding stop solution to stop the reaction

 

2. Materials Provided

	Reagent	Volume 96 Tests/192Tests
1	Brucellosis-Ag Coatedplate	1ea/2ea
2	Negative Control	1.5 ml/2.5 ml
3	Positive Control	0.5 ml/1 ml
4	Sample diluents	6/12ml
5	Washing solution (10X concentrated)	100ml
6	Enzyme conjugate	6/12ml
7	Substrate	11/22ml
8	Stopping solution	6/11ml
9	Adhesive plate sealer	2ea/4ea
10	Instruction	1 pcs

 

3. Material required not provided

1) Micropipette: 10ul-100ul, 100ul-1000ul.

2) Disposable pipette tips.

3) Cylinder: 500ml.

4) Microplate Reader: 96 wells.

5) Distilled water or deionized water.

6) Microplate Washer

 

4. Sample preparation

Take animal whole blood, get serum by using regular method, the serum should bright and no hemolysis

 

5. Washing buffer preparation

Return 10X Concentrated washing buffer into room temperature before use, if  there is salt crystals, shake to make it dissovled, then dilute it at 10 times with distilled water or deionized water. The diluted washing buffer can store at 4℃ for about 1 week.

 

 

  Interpretation of the Results

 

  Set zero at blank control well, read the OD value at 450nm (630nm as reference).

   For the assay to be valid:

   OD value of negative control(N) > 0.5, meanwhile positive value (P) blocking rate >80%

   Calculate method:

   PI(blocking rate)= {1- (Sample OD value/ Negative control OD average value)}x 100%

   Results interpretation

   PI(blocking rate)> 70%: Positive

   PI(blocking rate)≤ 70%: Negative

 

   Storage and expire date

    Store at 2~8℃ in dark, no frozen, expiry date: 12 months.

 

 

   

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