2xSYBR qPCR master Mix high ROX, suitable for ABI Prism7000/7300/7700/7900,Eppendorf,ABI Step One/Step One Plus

This qPCR Mix (Hig ROX) is a convenient premixed 2x concentrated solution for SYBR Green I-based qPCR which include HotStar Taq DNA polymerase, PCR buffer, dNTPs, SYBR Green I fluorescent dyes, Mg2+. It can be applied to the target sequence detection of genomic DNA and cDNA. The SYBR Green I dye binds to dsDNA without using sequence-specific probes. The modified HotStar Taq DNA polymerase is inactive under 40°C which effectively avoids the non-specific amplification caused by primer dimers or the nonspecific binding of prime and template. The unique PCR buffer components and the hot start enzyme ensure PCR specificity and sensitivity. The ROX is a dye molecule that can be used to normalize the well-to well fluorescent fluctuations and can be applied to all qPCR instruments using ROX as calibration dye. It has a higher sensitivity and a wider linear range which can exactly quantitate the target gene or low copy template.

This 

The SYBR qPCR Mix already include a high concentration rox, which is suitable for ABI Prism7000/7300/7700/7900, Eppendorf, ABI Step One/Step One Plus and so on.

Related Products

Roche LightCycler 480, Roche LightCyler 96, Bio-rad iCyler iQ, iQ5, CFX96 and some other thermol cycler do not require ROX correction  can choose The 2xSYBR qPCR Mix without Rox

ABI Prism7500/7500 Fast,QuantStudio® 3 System,QuantStudio® 5 System,QuantStudio® 6 Flex System,QuantStudio® 7 Flex System,ViiA 7 system,Stratagene Mx3000/Mx3005P,Corbett Rotor Gene 3000 and some other thermol cycler need a low concentration rox as a correction dye. You can choose our 2xSYBR qPCR Mix with (low Rox)

ABI Prism7000/7300/7700/7900, Eppendorf, ABI Step One/Step One Plus and etc. need a high concentration rox as a correction dye. You can choose our 2x SYBR qPCR mix with High Rox.

Except above we also offer the SYBR qPCR mix with seperate Rox, then you can add rox by yourself and can meet all the machine you want.

Application

Gene expression analysis
Gene copy number analysis

Applicable for Roche LightCycler 480,Roche LightCyler 96,Bio-rad iCyler iQ,iQ5,CFX96 and other machine  do not need Rox  correction.

Applicable for ABI Prism7000/7300/7700/7900, Eppendorf, ABI Step One/Step One Plus and so on

Features

High sensitivity: exactly quantitate the low copy template
High specificity: maximatily reduce the primer dimers and non-specific product
Wide linear range; Accurate quantitation

Aftersale Service

.ISO 9001:2000 certifi ed Quality Management System
International Authorized Third-Party Test For The Products You Demand.
Free lot-specifi c qualifi cation samples
Individual delivery schedules
Custom testing and documentation
Custom formulations, packaging & labeling
Free lot-specifi c qualifi cation samples
Service
1.Fast respond ,any inquiries will be replied within 12 hours.
2. Strong commitment and dedication to quality and service.
3. Customer requirment is the commercial information for both of us, we will kept strictly confidential
4. OEM/ODM Available.
5. Reasonable & competitive price, fast lead time.

Note

1) The concentration of the primer should be 0.1-1.0 µM and 0.2 µM is the best. Increase the concentration of primer when amplification is not high. Reduce the concentration when non-specific reaction appeared.
2) The recommended DNA template amount is between 10-100 ng of genomic DNA or 1-10 ng cDNA. As the target gene copy numbers are different among species, the template can be gradiently diluted to get an optimal template amount.

2. PCR reaction conditions:
Two step PCR
94℃ 2-3 min
94℃ 5-10 sec
60℃ 30-34 sec

Dissociation Stage
Tri step PCR
94℃ 2-3 min
94℃10-20 sec
55-60℃ 10-20 sec 40 cycles
72℃ 20-30 sec

Dissociation Stage

Our Company

Tinzyme is a professional molecular bio reagent Manufacture in China.The key products are nucleic acid substances and the enzymes/ Polymerase for molecular biology. We have many patents for the the nuceic acid and the enzymes. And the monomers of the nucleic are all enzymatically synthesized using our proprietary unique technology.These dNTPs and NTPs are functionally approved for all PCR- and related reactions including Real Time PCR, in vitro transcription assays and DNA-sequencing in diagnostics and molecular biology. Also the enzymes such as Taq DNA polymerase, M-MLV Reverse Transcriptase, pfu DNA polymerase are all worked very well. The perfomrace of our products can compared to the top bio companys of the world.