TransGen Biotech TransNGSTM rRNA Depletion Kit (Human/Mouse/Rat) for Next Generatione Sequencing/NGS on Illumina Platform

TransNGS® rRNA Depletion Kit (Human/Mouse/Rat)

Cat. No. KD101

Storage: at -20oC for one year

Description

TransNGS® rRNA Depletion Kit (Human/Mouse/Rat) depletes ribosomal RNA from human/mouse/rat total RNA by RNase H digestion, while retains messenger RNA and non-coding RNA. The depleted ribosomal RNA contains both cytoplasmic ribosomal RNA (5S rRNA, 5.8S rRNA, 18S rRNA and 28S rRNA) and mitochodrial ribosomal RNA (12S rRNA and 16S rRNA). The
 resulting rRNA-depleted RNA is suitable for RNA-Seq, random-primed cDNA synthesis, or other downstream RNA analysis applications.

• The kit can remove up to 99% ribosomal RNA from human/mouse/rat total RNA.

• Control qPCR Primer Sets are provided to monitor the depletion efficiency of ribosomal RNA and the retention rate of non-ribosomal RNA.

Sample Requirement

• Human/ Mouse/ Rat total RNA samples (10 ng-1 μg).

• This kit is suitable for both intact and degraded RNA (e.g. FFPE RNA).

Kit Contents