Virus DNA/ RNA Extraction Kit for blood immune organs dust sewage samples

Test Procedure

1. Sample preparation

(1)   Blood

Prepare serum/plasma samples.

(2) Tissue organs

Take 0.1 g sample(About the size of a soybean) to 2 mL centrifuge tube, then add 1 mL sterile saline, grinding and mixing, then vortex for 5 minutes with 8000×g, take out the supernatant for DNA extraction.

(3) Environment sample and others

a. Dust: Use a cotton swab to swab the surface of the instrument, desktop and etc. Then add 500 uL sterile saline, and centrifuge at 8000×g for 5 minutes, take out the supernatant for DNA extraction.

b. Saliva and nasal mucus: take the oral swab or nasal swab into 500 uL sterile saline directly, and then take out the supernatant for DNA extraction.

c. Sewage: take some mixture of sewage as sample.

2. Test method

(1) Transfer 200 uL processed sample to 1.5 mL centrifuge tube, and add 500 uL Lysis Buffer. Vortex and mixing 30 seconds. Sitting at the room temperature for 5 minutes.

(2) Transfer the mixture into a Spin Column and centrifuge at 8000×g for 1 minute, then discard the flow-through.

(3) Add 500 uL Wash Buffer I into the Spin Column, centrifuge at 12000×g for 1 minute, then discard the flow-through.

(4) Add 500 uL Wash Buffer II into the Spin Column, centrifuge at 12000×g for 1 minute, then discard the flow-through.

(5) Repeat (4) for once if necessary.

(6) Centrifuge at 12000×g for 2 minutes.

(7) Take out the Spin Column and place to a new 1.5 mL centrifuge tube. Add 500 uL Elution Buffer to wash the Spin Column, and then incubate at the room temperature for 2 minutes.

(8) Centrifuge at 8000×g for 2 minutes and discard the Spin Column. Then the buffer in the centrifuge tube contains the DNA. Store the DNA sample at -20℃ if not be used immediately.

Our advantages

1. Founded in 2008

2. 200-300 people, 40% technicians

3. 45,000 square meters

4. Quick response rate

5. Same price but better quality and service

CONTACT

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Wechat/ Tel: 86-18331598295

Email: yangye(at)wdwkbio.com

Q1: Are you a manufacturer?
Yes, advanced automate machines and professional R&D team ensure the high quality, speedy delivery and large production capacity.
Q2: Can we put our logo/text on the products or package?
Yes, we can do OEM or even ODM. All of our products can be customized.
Q3: Can you provide free samples?
Yes, free samples are available.
Q4: How long is your production time?
Our production time is usually two weeks after receiving the deposit and four weeks for one container order quantity.
Q5: What is the MOQ?
Small quantity like 1 box is available.
Q6: How to make the payment?
By T/T, Western union and Alibaba trade assurance order is also acceptable.