Biological enzyme fermentation alpha arbutin and beta arbutin 99.99% HPLC skin lighting
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- Supplier: Changsha Zhongren Biotechnology. ltd.
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Product Overview
Description
Company Profile
Changsha ZhongRen Biotechnology Co., Ltd. has been committing to the research, development and production and sales of natural products for a longterm, which relying on modern GMP manufacturing plant of Hunan Herbal Pharmaceutical Company Limited.
The company has a strong product development team, standardized quality management system, improved sales and service network. We provide plant extracts' generation of processing business, and we also willing to work with domestic and international partners to fight for people's healthier and happier life with tireless efforts and contribution.
Alpha-arbutin Product Manual
1.Brief Introduction
Arbutin, also known as bearberry glycoside. It can inhibit the activity of human body tyrosinase to prevent the formation of melanin, thus reducing the skin pigment deposition, removing colour spots and freckles. Usually used as the main raw material for whitening cosmetics internationally.
Arbutin, also known as bearberry glycoside. It can inhibit the activity of human body tyrosinase to prevent the formation of melanin, thus reducing the skin pigment deposition, removing colour spots and freckles. Usually used as the main raw material for whitening cosmetics internationally.
Based on the spatial orientation of the glycosidic bond, arbutin is divided into alpha-arbutin and beta-arbutin. They have the exactly same molecular formula, but different spatial structure: the glycosidic bond goes in the opposite direction.
2.Whitening Mechanism
Alpha-arbutin is a tyrosinase inhibitor, which mainly blocks the synthesis of dopamine and dopamine quinone, so as to curb the production of melanin, and thus whitening skin. It not only has the elimination effect to the skin freckles, old age spots, chloasma, but also has quite effective to the acne, skin moist, skin healing after a burns.
3.Main Applications
Advanced cosmetics: α-arbutin can be made into skin cream, freckle cream and advanced pearl cream- brightening agent, which can not only beautify and protect skin- whitening, moisturizing, and firming, but also reduce inflammation and irritant. In addition, the main effect of α-arbutin is the skin lightening in European and American countries, which has brought about a youthful and beautiful appearance.
Raw material of burn medicine: α-arbutin is the main ingredient of new burn medicine, which is characterized by quick pain relief, strong anti-inflammatory effect, rapid elimination of redness and swelling, quick healing and no scar.
Dosage form: spray or apply.
Raw material of burn medicine: α-arbutin is the main ingredient of new burn medicine, which is characterized by quick pain relief, strong anti-inflammatory effect, rapid elimination of redness and swelling, quick healing and no scar.
Dosage form: spray or apply.
4.Production Process, Water-Solubility Test and Optical Rotation Measurement
Alpha-arbutin is a new whitening material after Beta-arbutin. The original source is narrow, and can only be used by enzymes of different microorganisms to conduct glycolytic transfer reaction, combining one molecule of hydroquinone with one molecule of glucose to form a single alpha-arbutin. The enzyme synthesis method meets the requirements of environmental protection and has high stereoselectivity. Because the production process involves the cultivation of microorganisms to produce enzymes that catalyze glycosyl transfer reactions, the production process is also called fermentation. The arbutin synthase we selected belongs to the solid phase enzyme and can be recycled.
Alpha-arbutin is a new whitening material after Beta-arbutin. The original source is narrow, and can only be used by enzymes of different microorganisms to conduct glycolytic transfer reaction, combining one molecule of hydroquinone with one molecule of glucose to form a single alpha-arbutin. The enzyme synthesis method meets the requirements of environmental protection and has high stereoselectivity. Because the production process involves the cultivation of microorganisms to produce enzymes that catalyze glycosyl transfer reactions, the production process is also called fermentation. The arbutin synthase we selected belongs to the solid phase enzyme and can be recycled.
The Optical Rotation Measurement of Alpha-arbutin：
Instruments: Optical instruments, optical tubes-2 decimeters;
Determination: Test-product solution: 1.5g of test-product was accurately weighed, transferred to a 50ml volumetric flask, dissolved in water and diluted to the scale, shaken well (filtered by a 0.45 micron microporous filter membrane when necessary), and measured its rotatory power at 20±0.5℃ on a polarimeter for three consecutive times. Specific rotation was calculated by taking the average value.
Computing: Specific rotation = alpha * 50 / (w * 2 h (100%)) type, ɑ are averages of polarimetric reading: 2 for the optical tube length, dm: w as the weight of the sample, g: h for the dry weight loss of the sample.
Standard: For dry products, specific curl: between +175° and +185°.
Determination: Test-product solution: 1.5g of test-product was accurately weighed, transferred to a 50ml volumetric flask, dissolved in water and diluted to the scale, shaken well (filtered by a 0.45 micron microporous filter membrane when necessary), and measured its rotatory power at 20±0.5℃ on a polarimeter for three consecutive times. Specific rotation was calculated by taking the average value.
Computing: Specific rotation = alpha * 50 / (w * 2 h (100%)) type, ɑ are averages of polarimetric reading: 2 for the optical tube length, dm: w as the weight of the sample, g: h for the dry weight loss of the sample.
Standard: For dry products, specific curl: between +175° and +185°.
Additional Remark:
（1）Origin: The arbutin extracted from natural bearberry is not real alpha-arbutin, because alpha-arbutin can not be found in nature;
（2）Identification: Alpha-arbutin and beta-arbutin are differential isomers with very close physical and chemical properties. The simplest and most direct way to judge whether a product is alpha-arbutin is to test its optical rotation. The optical rotation of alpha-arbutin is positive (+174.0 ~ +184.0°), while the optical rotation of beta-arbutin is negative (-64° ~ -68°).
（3）Thermal Stability: After reaching 100℃, alpha-arbutin remained stable and hydroquinone was not detected. And beta-arbutin shows instability when reaching 60℃, which will decompose to produce hydroquinone. So alpha-arbutin is more resistant to high temperatures than beta-arbutin;
（4）Acid and Alkali Effect: Under neutral condition, alpha-arbutin is more stable.
（5）Toxicity and Side Effects: The hydroquinone (toxic) content in alpha-arbutin is almost no residue, while the hydroquinone content in beta-arbutin is much higher than that in alpha-arbutin. The amount of beta-arbutin added in cosmetics is about 3%, less than which has a weak inhibitory effect on the catalytic activity of tyrosinase in the skin and no obvious whitening effect. However, greater than this amount can significantly inhibit or damage cell growth, thus producing cytotoxicity, and its toxicological effects cannot be ignored. Studies have shown that alpha-arbutin inhibits only melanoma tyrosinase, and the mechanism is presumed to be mixed inhibition, while the mechanism of beta-arbutin is non-competitive. At a lower concentration, alpha-arbutin can effectively inhibit melanin, and at the same time, alpha-arbutin does not inhibit cell growth, so it is obvious that alpha-arbutin has higher safety than beta-arbutin.
Note: Neither alpha-arbutin nor beta-arbutin can be taken orally.
7. The Contrast of Alpha-arbutin and Phloretin (Apple Extract)
(1)Whitening Mechanism: Same. It can effectively inhibit the activity of tyrosinase in the skin and block the formation of melanin, thus resulting in whitening effect.
(2)Process Difference: Similar. All are prepared by enzyme fermentation.
(3)Water Soluble: Alpha-arbutin is more soluble in water than phloretin; The water solubility of phloretin is very poor.
(4)Stability: Phloretin is unstable under alkaline, neutral and strong oxidation conditions, but stable under acidic environment, strong light and high temperature. Different antioxidants have certain protective effects on its stability. Arbutin is easy to decompose in acidic environment. Please pay attention to the neutral pH of cream and emulsion, which is controlled at 5-7.
(5)Termination Usage: Phoridin can be used to develop functional cosmetics with antibacterial and antiseptic properties. It has strong antioxidant ability and can effectively delay skin wrinkles, aging and other aging symptoms. Alpha-arbutin can be used as a skin lightener, and is also very effective for moisturizing the skin, healing after skin burns and acne.
（2）Identification: Alpha-arbutin and beta-arbutin are differential isomers with very close physical and chemical properties. The simplest and most direct way to judge whether a product is alpha-arbutin is to test its optical rotation. The optical rotation of alpha-arbutin is positive (+174.0 ~ +184.0°), while the optical rotation of beta-arbutin is negative (-64° ~ -68°).
（3）Thermal Stability: After reaching 100℃, alpha-arbutin remained stable and hydroquinone was not detected. And beta-arbutin shows instability when reaching 60℃, which will decompose to produce hydroquinone. So alpha-arbutin is more resistant to high temperatures than beta-arbutin;
（4）Acid and Alkali Effect: Under neutral condition, alpha-arbutin is more stable.
（5）Toxicity and Side Effects: The hydroquinone (toxic) content in alpha-arbutin is almost no residue, while the hydroquinone content in beta-arbutin is much higher than that in alpha-arbutin. The amount of beta-arbutin added in cosmetics is about 3%, less than which has a weak inhibitory effect on the catalytic activity of tyrosinase in the skin and no obvious whitening effect. However, greater than this amount can significantly inhibit or damage cell growth, thus producing cytotoxicity, and its toxicological effects cannot be ignored. Studies have shown that alpha-arbutin inhibits only melanoma tyrosinase, and the mechanism is presumed to be mixed inhibition, while the mechanism of beta-arbutin is non-competitive. At a lower concentration, alpha-arbutin can effectively inhibit melanin, and at the same time, alpha-arbutin does not inhibit cell growth, so it is obvious that alpha-arbutin has higher safety than beta-arbutin.
Note: Neither alpha-arbutin nor beta-arbutin can be taken orally.
7. The Contrast of Alpha-arbutin and Phloretin (Apple Extract)
(1)Whitening Mechanism: Same. It can effectively inhibit the activity of tyrosinase in the skin and block the formation of melanin, thus resulting in whitening effect.
(2)Process Difference: Similar. All are prepared by enzyme fermentation.
(3)Water Soluble: Alpha-arbutin is more soluble in water than phloretin; The water solubility of phloretin is very poor.
(4)Stability: Phloretin is unstable under alkaline, neutral and strong oxidation conditions, but stable under acidic environment, strong light and high temperature. Different antioxidants have certain protective effects on its stability. Arbutin is easy to decompose in acidic environment. Please pay attention to the neutral pH of cream and emulsion, which is controlled at 5-7.
(5)Termination Usage: Phoridin can be used to develop functional cosmetics with antibacterial and antiseptic properties. It has strong antioxidant ability and can effectively delay skin wrinkles, aging and other aging symptoms. Alpha-arbutin can be used as a skin lightener, and is also very effective for moisturizing the skin, healing after skin burns and acne.
9.Volume of Addition Suggested
Alpha-arbutin: 0.2%-5% (facial mask 0.5%-1%, cream 2%)
Beta-arbutin: 1%-7%
The following matters should be paid attention to when preparing skin care products:
★ Arbutin is easy to decompose in acidic environment, pay attention to cream emulsion system pH control at 5-7;
★ Dissolve arbutin in a small amount of 50℃ water, and added at 50℃ after cream emulsification;
★ Adding natural vegetable oil containing oleic acid and linoleic acid can promote arbutin synergistic effect;
★ Adding 0.8 -1.0% of the azone in the cosmetics system, can promote the absorption of arbutin and prevent arbutin in the skin precipitation at the same time.
Beta-arbutin: 1%-7%
The following matters should be paid attention to when preparing skin care products:
★ Arbutin is easy to decompose in acidic environment, pay attention to cream emulsion system pH control at 5-7;
★ Dissolve arbutin in a small amount of 50℃ water, and added at 50℃ after cream emulsification;
★ Adding natural vegetable oil containing oleic acid and linoleic acid can promote arbutin synergistic effect;
★ Adding 0.8 -1.0% of the azone in the cosmetics system, can promote the absorption of arbutin and prevent arbutin in the skin precipitation at the same time.
10.Products of the Market
11.Package: & Storage
Package:
1 to 5 Kilo: Ordinary plastic sealed bag inside with aluminum foil bag and paper box outside.
More than 5 Kilo: Two layers PE bag with paper bucket (Round and square) or paper bucket with iron ring.
Storage:
In cool & dry place, keep away from strong light and heat.
1 to 5 Kilo: Ordinary plastic sealed bag inside with aluminum foil bag and paper box outside.
More than 5 Kilo: Two layers PE bag with paper bucket (Round and square) or paper bucket with iron ring.
Storage:
In cool & dry place, keep away from strong light and heat.
Quality Assurance
Our advantages
- Highest raw materials.
- GMP factory.
- Advanced production technology.
- Advanced testing equipment.
- Annual output: 300 tons.
Packing & Delivery
FAQ
1. How to contact with us ?
a. Online service: By Trade manager, Skype, Whatsapp, Wechat, QQ.
b. Send inquiry to us or email us.
c. Call us without any hesitation.
2. How to confirm the product quality before placing orders ?
a. We will offer you our products specification standard to you, and we test every batch of our product by HPLC, UV, GC and TLC and etc. We also cooperate with independent third party lab such as SGS, Eurofins etc.
b. Free samples of some products can be sent, only need to pay the shipping cost or arrange a courier to us and take the samples.
c. Send us your products specifications and requests, we will confirm it.
3. How to make order & payment ?
a. Proforma invoice will be sent after your confirmation of the order, or you can order in Alibaba.
b. We can accept payment by T/T, Western Union, Paypal, Bank of China L/C.
4. How to pack and storage ?
a. Packing: 1kg/Aluminum foil bag with Carton outside.
25kg/Fiber drum with double PE bags inside.
b. Storage: Store in cool & dry place, keep away from strong light and heat.
5. How to ship and delivery ?
a. Transit could be DHL,UPS,TNT, FEDEX, EMS. For mass orders, it will be delivered by air or sea.
b. Depending on your location,
For small order, please expect 5-7 days by DHL,UPS,TNT, FEDEX, EMS.
For mass order, please allow 5-8 days by Air, 20-35 days by Sea. cbd oil Organic Cbd ginseng extract cbd isolate hemp oil beer powderPowder Thc thc oil natural Moringa soy peptide water soluble aloe vera geltadanafil powder almond flour Ephedra turmeric powder Stevia 99.9% cbd isolatemanjakani extract red tongkat ali extract ginseng extract sakura extract gambier extract black walnut extract ganoderma extract sakura extract powder pure kudzu extract horseradish extract
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