The essential reagents required for an immunoassay include high affinity and specificity antibodies (enzyme
conjugated and immobilized), with different and distinct epitope recognition, in excess, and native antigen. In this
procedure, the immobilization takes place during the assay at the surface of a microplate well through the interaction of antibody to FITC coated on the well and added FITC labeled monoclonal anti- ß HCG antibody. Upon mixing FITC labeled antibody, the enzyme labeled antibody and a serum containing the native antigen, reaction results between the native antigen and the antibodies, without competition or stearic hindrance, to form a soluble sandwich complex.
The interaction is illustrated by the following equation:.
Simultaneously, the complex is deposited to the well through the high affinity reaction of FITC and antibody to FITC. After equilibrium is attained, the antibody-bound fraction is separated from unbound antigen by decantation or aspiration. The enzyme activity in the antibody-bound fraction is directly proportional to the native antigen concentration. By utilizing several different serum references of known antigen value.
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ExpoMedical exhibition in Argentina Date: from September 26, 2018 to September 28, 2018 Add.: Centro Costa Salguero, Buenos Aires, Argentina Our booth No: C12 in Hall 4
MEDICA in Germany Date: from November 12th to November 15th Add.: Messe Düsseldorf Out booth No: will update in June
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