GDSBIO Taq DNA Polymerase PCR reagent 5U/Microliter 100000U P1017

Product Overview

Description

Description

Taq DNA Polymerase is a thermostable recombinant DNA polymerase derived from thermophilic bacterium Thermus aquaticus. Its molecular weight is 94 kDa. Taq DNA Polymerase can amplify DNA target up to 5 kb(simple template). The elongation velocity is 0.9~1.2kb/min (70~75°C). It has 5' to 3' polymerase activity but lacks of 3' to 5' exonuclease activity that results in a 3'-dA overhangs PCR product.

 

Unit Definition

One unit is defined as the amount of the enzyme required to catalyze the incorporation of 10 nmole of dNTPs into an acid-insoluble form in 30 minutes at 70°C using hering sperm DNA as substrate.

 

Storage Buffer

20mMTrisCl ( pH8.0), 100mM KCl, 3.2mM MgCl₂ 1mM DTT,0.1% Triton X-100 ,0.1% Tween20, 0.2mg/ml BSA, 50% (v/v) glycerol

 

10X PCR Buffer with Mg²⁺

100mMTris-HCl(PH 8.8), 500mMKCl, 1%Triton-X-100, 16mM MgCl₂

 

Applications

• PCR amplification of DNA fragments as long as 5 kb

• DNA labeling.

• DNA sequencing.

• PCR for cloning.

 

Quality Control

The absence of endodeoxyribonucleases, exodeoxyribonucleases and ribonucleases is confirmed by appropriate quality tests. Functionally tested in amplification of a single-copy gene from human genomic DNA.

 

Supply

Small package or Bulk order

 

 Store all components at –20°C

 

Dongsheng Biotech PCR Reagents are looking for distributors and providing OEM service

 

Dongsheng Biotech offers different series of products to help you achieve PCR success. For enzymes, our Taq Polymerase, HS Taq DNA Polymerase, FS Taq DNA Polymerase, Pfu DNA Polymerase and Fusion Pfu DNA Polymerase provide high fidelity, efficient, sensitivity PCR performance. We also have Long Taq DNA Polymerase for long PCR performance.

 

 

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Dongsheng Biotech PCR Reagents are looking for distributors and providing OEM service

Dongsheng Biotech offers different series of products to help you achieve PCR success. For enzymes, our Taq Polymerase, HS Taq DNA Polymerase, FS Taq DNA Polymerase, Pfu DNA Polymerase and Fusion Pfu DNA Polymerase provide high fidelity, efficient, sensitivity PCR performance. We also have Long Taq DNA Polymerase for long PCR performance.