SYBR Green qPCR Mix (High Rox ), chemistry dye real time pcr, P2091b 1ml

Product Overview

Description

Cat. No: P2091b, 400 rxn/20μl reaction

P2092b, 2000 rxn/20μl reaction

 

SYBR^® Green qPCR Mix (High ROX+)

For research use only

Components  

Component	P2091b	P2092b
2X SYBR® Green qPCR Mix (High ROX+)	1 ml	1 ml × 5
Nuclease-free Water	1 ml	1 ml × 5

 

Storage

This reagent can be stored for 2 months at 4°C and protected from light. For longer storage, it should be kept at -20°C and protected from light.

Description

2x SYBR^® Green qPCR Mix (High Rox⁺) is designed for high-performance, high-throughput, real-time PCR. The kit contains a Taq DNA polymerase engineered through a process of molecular evolution. The result is a unique enzyme, specifically designed for qPCR using SYBR^®Green I dye chemistry.

 

2x SYBR^® Green qPCR Mix (High Rox⁺) is a convenient premix of the components (except primers, template, and water) necessary to perform real-time polymerase chain reaction (PCR) using SYBR^®Green I dye with enhanced sensitivity and specificity. The SYBR^®  Green dye binds to double-stranded (ds) DNA, thus providing a fluorescent signal that reflects the amount of dsDNA product generated during PCR. This reagent is used in amplification and detection of DNA in qPCR on ABI real-time instruments that support normalization with high Rox reference dye at a final concentration of 500 nM.

 

Applications

n            Gene expression analysis

n            Low copy gene detection

n            Microarray validation

n            Gene knockdown validation

 

Features

n            This reagent can be used in ABI Real-time systems that require high concentration of Rox reference dye.

n            Hot Start technology with anti-Taq DNA polymerase  antibodies enables high specificity and reproducible amplification.

 

Composition of the SYBR Green qPCR Mix

100 mM KCl , 5 mM MgCl2, 400 μM dNTPs, 0.1 U/μl Hot Start Taq DNA Polymerase, 1x SYBR® Green, 2% Rox reference dye and other optimized buffer components.

 

 

 

 

 

 

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Dongsheng Biotech PCR Reagents are looking for distributors and providing OEM service

 

 

 

Dongsheng Biotech offers different series of products to help you achieve PCR success. For enzymes, our Taq Polymerase, HS Taq DNA Polymerase, FS Taq DNA Polymerase, Pfu DNA Polymerase and Fusion Pfu DNA Polymerase provide high fidelity, efficient, sensitivity PCR performance. We also have Long Taq DNA Polymerase for long PCR performance.