Cholinesterase Biochemistry Reagent Manufacturers Best Quality Diagnostics Kits With Best Price From India

 CHOLINESTERASE

Intended Use:

Test for quantitative estimation of Cholinesterase activity in human serum and plasma.

Introduction:

There are two forms of cholinesterase; acetyl cholinesterase and cholinesterase which is also commonly referred to as psuedocholinesterase. Acetylcholinesterase is found predominantly in erythrocytes. Cholinesterase is synthesised in the liver and is present in plasma and is the form of the enzyme routinely measured. Cholinesterase is most commonly measured as an indicator of exposure to anticholinesterases (organophosphates, including many insecticides), or inherited abnormal variants of the enzyme, which cause a decreased level of plasma cholinesterase. Increased levels of activity may be present in nephrotic syndrome or in the recovery from liver damage. Clinical diagnosis should not be made on a single test result; it should integrate clinical and other laboratory data.

Principle:

Butyrylthiocholine iodide is hydrolysed by cholinesterase to produce thiocoline in the presence of potassium hexacyanoferrate (III), the absorbance decrease at 405nm is directly proportional to the cholinesterase activity in the sample.

Features:

üMethod Specific for Cholinesterase

üLiquid Stable Reagents with Long Shelf Life

üFast Result – 2 minutes per sample

üHigh Linearity

 Specification:

Methodology: Butyrylthiocoline Potassium Hexacynoferrate (Iii)

Reaction Type: Kinetic

Reaction Direction: Decreasing

Sample Type: Human Serum or Plasma

Number of Reagents: 2

R1:R2 Mixing Ratio: 4:1

Primary Wavelength: 405 nm

Secondary Wavelength: NA

Flow Cell Temperature: 37 ⁰C

Blank Settings: Distilled Water

Blank Absorbance Limit: >0.800

Reagent Volume Required Per Test: 1000 µl

Sample Volume Required Per Test: 15 µl

Delay Time: 60 sec

Read Time: 60 sec

Incubation Temperature: 37 ⁰C

Calibrator Available in the Kit: No

Calibrator Concentration: NA

Factor: 73,000

Linearity: 12000 IU/L

Automation: Enabled

Pack Size:

25 ml – 1x20 ml + 1x5 ml

Dispatch time in weeks:

2 to 4 weeks.