Storage Condition and Valid Period

ProteinaseKshouldbestoredat-20°C±5°C,othercomponentscanbestoredat0-35°C, and the shelf life is 12months.

We suggest products transportation at 0-40°C for no more than 7 days.

Sample Requirements

Applicable Samples:Whole blood, tissue homogenate, swab, serum, plasma, bronchoalveolar lavage fluid and other cell-free body fluids.

Procedure

Equipment and Reagents to Be Supplied by User:Constant temperature mixer  isopropanol.

Things to do before starting: Mix all the reagents and gently invert 3-5 times before use.

1.      Take 1.5 ml centrifugal tube (supplied by user), add 20 μl protease K (the sample needstobebalancedtoroomtemperature),200μlsample,200μllysisbuffer,300 μl isopropanol. Vortex for 5 seconds, and store it at room temperature and vortex for 10 minutes with a constant temperature mixer of 1200rpm.

Notes:Forwetswab,take200μlsample,mixitthoroughly.Soakthedryswabsamplein400μl normal saline and mix well, let stand for 5 minutes, centrifugate at 12000 rpm for 1 minute, and extract 200μl.

2.      The obtained solution from step 1 was transferred to the adsorption column of collection tube. Centrifugation at 12000 RPM (~ 13400 g) for 1 minute, and discard the liquid. And then put the adsorption column back into the collectiontube.

3.      Add500μlwashingbuffer1totheadsorptioncolumn,centrifugateat12000rpmfor 1minute,discardtheliquid,andputtheadsorptioncolumnbackintothecollection tube.

4.      Add500μlwashingbuffer2totheadsorptioncolumn,centrifugateat12000rpmfor 1minute,discardtheliquid,andputtheadsorptioncolumnbackintothecollection tube.

5.      Centrifugationat12000rpmfor2minutes,anddiscardwasteliquidinthecollection tube. Place the column at room temperature for 2 minutes and let itdry.

6.      Add 40-100 μl RNase-Free water to the middle part of the adsorption column membrane,keep 2 minutes at room temperature and centrifugate for 1 minute at 12000 rpm, the RNA solution was preserved at -80°C to preventdegradation.

Note

1.      Please read this manual carefully before doingexperiment.

2.      Please check whether there is crystallization or precipitation in the lysis buffer before use. If it happens, please dissolve it in a 56℃water bath.

Store :RNase A: store at -20°C.

Before starting, please add RNase A to Buffer PI, mix well and store at 4°C.

Other reagent can be store at room temperature.

If precipitate forms in the buffers during storage, it should be redissolved by incubating the buffers at 37°C before use.

Packaging size:

50 preps/ box  18boxes per carton
100 preps/ box  18boxes per carton