Набор ДНК дрожжей TIANamp для извлечения дрожжей ДНК для очистки нуклеиновой кислоты

Product Overview

Description

—— Extraction of high quality genomic DNA from various yeast

 

Cat. No:
4992449

 

Packing Size:
50 preps

 

 

 

Contents	50 preps
Buffer GA	15 ml
Buffer GB	15 ml
Buffer GD	13 ml
Buffer PW	15 ml
Buffer TE	15 ml
Proteinase K	1 ml
Spin columns CB3	50
Collection tubes (2 ml)	50

 

Storage:
Room temperature (15-25℃)

 

Description:

The TIANamp Yeast DNA Kit adopts a highly efficient DNA-binding spin column and a unique buffer system to extract yeast genomic DNA. After the sample is lysed, the DNA specifically binds to the silica membrane under high salt condition. The DNA then elutes from the silica membrane under low salt and high pH condition. The DNA purified by this kit can be applied in various tests, including restriction digestion, PCR and Southern blot.

 

Required Reagents:

Sorbitol buffer, lyticase, ethanol, RNase A (optional)

 

Features:

• Short operation time: High quality yeast gDNA could be obtained within one hour.


• Non-toxic: The operation is safe since no phenol or chloroform extraction is needed.


• High purity: Pure DNA can be obtained by using this kit, which can be directly used in downstream molecular biology experiments such as PCR, restriction digestion, Southern blotting, etc.

 

Applications:

• PCR and qPCR.


• Restriction digestion, library construction, Southern blotting