diagnostics Beta lactams and sulfonamides and Tetracyclines triple rapid test strip for milk

Beta-lactams and sulfonamides and Tetracyclines triple rapid test strip for milk

1. The test principle 

This kit is based on the specific reaction of antibody-antigen and immunochromatography. β-lactams and sulfonamides and tetracyclines antibiotics in the sample compete for the antibody with the antigen coated on the membrane of the test strip. Then after a color reaction, the result can be observed.

 2.Applications

This kit is used for rapid qualitative analysis of β-lactams and sulfonamides and tetracyclines in milk sample.

 3.Kit component

 4.Detection limit

5.Assay steps

(1)     Read the instructions carefully before experiment. Bring the test kit and samples to room temperature. Milk samples should be fully liquid without any agglomeration or deposition.

(2)     Take bottles needed from the kit package, take out required wells and strips, and make proper marks. Please use these test strips within 1h. Seal the cap of the bottles. The rest strips can be stored for future use.

(3)     Preheat the metal incubator to 45℃.

(4)     Place the mircrowells in the metal incubator, Take 200ul of the test samples into the wells, then repeatedly absorb and drop for 5 times to mix the sample with the reagent in the wells completely. The mixture should be pink, and then start the timer.

(5)     Incubate for 3min in the metal incubator (45℃); insert the test strips into the wells with the "Absorbent pad" end fully dipped in to the mixture.

(6)     Incubate for 6minin the metal incubator(45℃) again. Take out the strip; judge the result according to Part 7.

              Notice: please read the results in five minutes, this result will be valid.

6.Test results

There are 4 lines in the dipstick, Control line, Beta-lactams Line, Sulfonamides Line and Tetracylcines Line, which are briefly used as “C”, “T₁”,”T₂” and “T₃”. The test results will depend on the color of these lines. The following diagram describes the result identification.

7.1 Method 1(manual interpretation)

Negative: Line C, Line T₁ ,LineT₂ and Line T₃ are all red, color of Line T₁ ,Line T₂ and Line T₃are all darker than or similar to Line C, indicating the corresponding residue in sample is less than LOD of the kit.

Beta-lactams Positive: Line C is red, color of Line T₁is weaker than Line C, indicating the beta-lactams residue in sample is higher than LOD of the kit.

Sulfonamides Positive: Line C is red, color of Line T₂is weaker than Line C, indicating the sulfonamides residue in sample is higher than LOD of the kit.

Tetracyclines Positive: Line T is red, color of Line T₃is weaker than Line C, indicating the tetracyclines residue in sample is higher than LOD of the kit.

Please notice: Control line is used as a quality indicator, which will always appear regardless of the B/T line. If Control line does NOT appear, this indicates that the result is invalid. Users please check the kit insert again and re-do the assay with new test dipstick.

7.Storage

 2-8℃ in cool dark place, do not freeze. The kit will be valid in 12 months. 

Ailsa Wang

Email: wangna(at)kwinbon.com.

Wechat:wn2014012209

Skype/WhatsApp: +86 15231118512

Company: Beijing Kwinbon Biotechnology Co.,Ltd

Payment Terms

All orders are to be 100% prepaid in advance. We accept bank transfer, western union, etc.

Shipment

All products from Kwinbon Biotech will be shipped by DHL, TNT, UPS or Fedex according to the customer's requirement. 

Package

The kits will be packed in foam box with ice package.

Certificate

ISO9001: 2008 & ISO13485: 2003; 

UKAS (United Kingdom Accreditation Service); 

CNAS (China National Accreditation Service for Conformity Assessment); 

CQC (China Quauty Certification Center); 

CE (Communate Europpene).

 Q1. Can I get the full catalog from your side ?

A1: Yes, please send your request to the official email.

Q2. Is Kwinbon a big company or a small company ?

A2: Kwinbon established in 2002 and now is China's Largest and strongest company in food safety products field. Kwinbon has more than 500 empolyees and 4 branch companies.

Kwinbon has over 10,000 square meters R&D laboratories, SPF animal house, and GMP factory. The R&D lab is equipped with instrument like GC, HPLC, LC-MS/MS. We also have 17 local sales offices.

Q3. Is Kwinbon professional ?

A3: Kwinbon has established close collaborations with China CDC, Association of Food Science and Technology, Chinese Academy of Agriculture Science, National Institute of Food Quality Supervision and Inspection, China Agricultural University. Among the staffs, there are more than 500 employees, including 18 doctors, 92 masters and 24 senior engineers. And also 46% of the employees are working on R&D.

Q4. Is Kwinbon certificated ?

A4: Kwinbon passed " ISO9001:2008 " and " ISO13485:2003";

Kwinbon also won the "2011 Fobes China Best SEMs" certificates;

Kwinbon won about 100 patents in food safety detection field.

Q5. Payment terms ?

A6: 100% T/T in advance, L/C, Western Union.