—— Rapid purification of high quality genomic DNA from various plant tissues with different starting amount according to specific experimental requirements
Cat. No: 4992709
Packing Size: 50 preps
Contents
50 preps
Buffer FP1
25 ml
Buffer FP2
10 ml
Buffer TE
15 ml
RNaseA(10 mg/ml)
300 μl
Storage:
Room temperature (15-25℃)
Description:
The DNAquick Plant System adopts a unique buffer system to extractgenomic DNA from a variety of fresh or cryopreserved plant tissues. Thiskit does not require phenol or chloroform extraction and is safe to operate.It can extract large fragments of genomic DNA with high purity and stablequality.
· Simple and fast: The kit is provided as liquid system, and high qualitypurified gDNA could be obtained within 1 hour from various plant tissuesrapidly.
· Non-toxic: The operation is safe since no phenol or chloroform extractionis needed.
· No limit for the starting amount of the materials: Users can adjustthe initial amount of the samples according to specific experimentalrequirements flexibly.
· High cost-effective: The good quality and popular price makes the kit verysuitable for large amount plant genome extraction.
Applications:
· PCR, qPCR and mutiplex PCR
· DNA profiling techniques such as RAPD, RFLP, AFLP, SSR, etc.
· Library construction, Southern blotting
DNA Extraction Yield From Various Plant Tissues:
Plant Sample
Sample Amount
Yield Range
OD260 / OD280
Arabidopsis thaiana
100 mg
3-5 μg
1.7 - 1.9
Wheat
100 mg
25-32 μg
1.7 - 1.9
Pine tree
100 mg
25-32 μg
1.7 - 1.9
Potato
100 mg
5-7 μg
1.7 - 1.9
Tomato
100 mg
12-16 μg
1.7 - 1.9
Rape
100 mg
2-5 μg
1.7 - 1.9
Tobacco
100 mg
20-30 μg
1.7 - 1.9
Rice
100 mg
12-30 μg
1.7 - 1.9
Soybean
100 mg
20-32 μg
1.7 - 1.9
Corn
100 mg
20-32 μg
1.7 - 1.9
Note: DNA yield depends on sample types. All the materials above are from tender leaves.
Experimental Example:
Genomic DNA isolated from 100 mg leaves of different plantsusing DNAquick PlantSystem.3 μl of 100 μl eluates were loaded perlane.
1: Fresh soybean leaves;
2: Fresh tobacco leaves;
3: Fresh wheat leaves;
4: Fresh corn leaves.
The concentration of the agarose gel was 1%. The electrophoresiswas performed under 6 V/cm for 20 min.
M: λDNA/Hind III Marker
Company Information
TIANGEN, a leading biotechnology company in China, provides customized molecular biology reagents and professional technical services to customers in the life science research field. Our comprehensive product portfolio includes technologies for Nucleic acid purification products, PCR series reagents ( including regular PCR, RT-PCR and Real time PCR), NGS library preps and NGS service, as well as lab essential instrumentations.
TIANGEN maintains a strict“Customer First”mentality and is dedicated to a continuous improvement in quality to fulfill our promise of best-in-class products and services.
