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Trans2K DNA Marker
Trans2K® DNA Marker is composed of six linear double-stranded DNA bands. This ready-to-use product is premixed with 1x DNA loading buffer. Based on experiment requirement, 5 μl marker can be directly loaded for gel electrophoresis. It provides simple operation and produces clear image. For easy reference on agarose gels, the 750 bp band (100 ng/5 μl) is two times brighter than the other bands (50 ng/5 μl) in the ladder.
| Cat. No. | BM101 | ||
| Specification | BM101-01 | 500 µl | |
| BM101-02 | 5×500 µl | ||
| Storage: | at 4oC for six months; at -20oC for two years | ||
| Concentration: | 0.07 mg/ml | ||
| Advantages | Clear bands, easy determination | ||
| Composition | 100 bp, 250 bp, 500 bp, 750 bp (100 ng/5 μl, the double intensity band), 1000 bp, 2000 bp | ||
| Storage Buffer | 10 mM Tris-HCl (pH 8.4), 10 mM EDTA, 0.02% bromophenol blue, 5% glycerol | ||
| Instructions for Use | Load Trans2K® DNA Marker on the lane on a 1.5% agarose gel (load 1 μl marker for 1 mm lane, increase the loading volume for wider lane ). | ||
| Suggested electrophoresis conditions: 1.5% agarose gel, voltage 4-10 v/cm. | |||
| Stained with EB or GelStain (GS101), visualize the bands under exposure to ultraviolet light. | |||
| Notes | Completely thaw and mix well before use. | ||
| Please change the electrophoresis buffer in time and use freshly-prepared gel to avoid adverse on the result. | |||
CITATIONS
Hu X, et al. 2013. Molecular Cloning and Functional Characterization of the Dual Oxidase (BmDuox) Gene from the Silkworm Bombyx mori. 8(8):e70118. PLoS One. IF=3.73. PMID: 23936382.
Wu SF. et al. 2013. Molecular cloning and pharmacological characterisation of a tyramine receptor from the rice stem borer, Chilo suppressalis (Walker). 69(1):126-34. Pest Manag Sci. IF= 2.594. PMID: 23129510.




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