TransGen Biotech TransScript Probe One Step qRT PCR SuperMix Real Time RT PCR Master Mix from Biotech Consumables Companies
- Category: >>>
- Supplier: TransGen Biotech Co. Ltd. [Beijing China]TransGen
Share on (756740817):
Product Overview
Description
TransScript Probe One-Step
qRT-PCR SuperMix
TransScript® Probe One-Step qRT-PCR SuperMix is designed for one-step qRT-PCR with high sensitivity, high synthesis efficiency and high amplification efficiency. This kit firstly synthesizes first-strand cDNA with RNA as templates using reverse gene-specific primers, and then performs qPCR with the synthesized cDNA as templates using both forward and reverse gene-specific primers and fluorescent probes to achieve one step from reverse transcription to qPCR in a single tube.
• Simple to use with reduced contamination
• High sensitivity and high specificity
| Cat. No. | AQ221 | |
| Specification | AQ221-01 | 100 rxns (20 μl per reaction) |
| AQ221-02 | 400 rxns (20 μl per reaction) | |
| Storage: | at -20°C for two years | |
| Shipping | Dry ice (-70 ℃) | |
| Advantages | Simple procedure helps to minimize contamination. | |
| High efficiency Enzyme. | ||
| • High sensitivity, high specificity, accurate data. | ||
| Applications | • High copy and low copy gene detection. | |
| •GC-rich templates or complex secondary structure RNA templates. | ||
| • Viral RNA and trace RNA detection. | ||
| Notes | • Avoid any RNase contamination. | |
| • High quality RNA template is recommended for use to ensure successful cDNA synthesis. | ||
| • This kit is only suitable for GSP, but unsuitable for first-strand cDNA synthesis using Oligo(dT) or Random Primer. | ||
Kit Contents
|
Component |
AQ221-01 |
AQ221-02 |
|
TransScript®One-Step RT/RI Enzyme Mix |
40 μl |
160 μl |
|
2×TransStart®Probe qPCR SuperMix |
1 ml |
4×1 ml |
|
Passive Reference Dye (50×) |
40 μl |
160 μl |
|
RNase-free Water |
1 ml |
4×1 ml |
Reaction Components
| Component | Volume | Final Concentration |
| RNA Template | Variable | as required |
| Forward GSP (10 μM) | 0.4 μl | 0.2 μM |
| Reverse GSP (10 μM) | 0.4 μl | 0.2 μM |
| Probe (10 µM) | 0.4 μl | 0.2 µM |
| 2×TransStart® Probe qPCR SuperMix | 10 μl | 1× |
| TransScript® One-Step RT/RI Enzyme Mix | 0.4 μl | - |
| Passive Reference Dye (50×) (optional) | 0.4 μl | 1× |
| RNase-free Water | Variable | - |
| Total volume | 20 μl | - |
Packaging
We Recommend
New Arrivals
New products from manufacturers at wholesale prices
