EasyScript Reverse Transcriptase [M MLV, RNase H ] RT Enzyme/ cDNA Synthesis Kit by TransGen Biotech

Product Overview

Description

EasyScript^® Reverse Transcriptase

[M-MLV, RNase H-]

EasyScript^® Reverse Transcriptase is an engineered version of M-MLV reverse transcriptase with deficient RNase H activity. The enzyme is purified to near homogeneity from E.coli containing the modified M-MLV RT gene.

 

Cat. No.	AE101
Specification	AE101-02	10000 units
	AE101-03	5×10000 units
Concentration:	200 units/μl
Storage:	at -20°C for one year
Advantages	Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNA synthesis.
	Anchored Oligo(dT)18 Primer for higher yield and more full length cDNA.
	cDNA up to 8 kb.
Applications	First-strand cDNA synthesis
	Multiple copy gene synthesis

 

Kit Contents

Component	AE101-02	AE101-03
EasyScript® RT	10000 U	5×10000 U
5×ES RT Buffer	200 μl	5×200 μl
Anchored Oligo(dT)18 (0.5 μg /μl)	50 μl	250 μl

 

 

 

 

 

 

 

First-Strand cDNA synthesis reactions

  1. Reaction Components

Component	Volume
Total RNA/mRNA	50 ng-5 μg/5-500 ng
Anchored Oligo(dT)18 Primer (0.5 μg /μl)	1 μl
or Random Primer(N9) (0.1 μg/μl)	1 μl
or GSP	2 pmol
10 mM dNTPs	1 μl
5×ES RT Buffer	4 μl
Ribonuclease Inhibitor (50 units/μl)	0.5 μl
EasyScript® RT	1 μl
RNase-free Water	to 20 μl

 

 

RT-PCR

Reaction Components

Component	Volume	Final Concentration
cDNA	2 μl	as required
Forward Primer (10 μM)	1 μl	0.2 μM
Reverse Primer (10 μM)	1 μl	0.2 μM
10×TransTaq® HiFi Buffer II (with Mg2+)	5 μl	1×
2.5 mM dNTPs	4 μl	0.2 mM
TransTaq® HiFi DNA Polymerase	0.5 μl	2.5 units
ddH2O	to 50 μl	-

 

 

 

CITATIONS

Xu P. et al. 2010. Molecular cloning and characterization of four heat shock protein genes from Macrocentrus cingulum (Hymenoptera: Braconidae). 37(5):2265-72. Mol Biol Rep. IF= 2.929.  PMID: 19685164.

 

Gao B. et al. 2011. Complete genomic sequence analysis of a highly virulent isolate revealed a novel strain of Sugarcane mosaic virus. 43(3):390-7. Virus Genes. IF= 1.845.  PMID: 21833715.

 

Jin F. et al. 2012. Synergistic antitumor effects of endostar in combination with oxaliplatin via inhibition of HIF and CXCR4 in the colorectal cell line SW1116. 7(10):e47161. PLoS One. IF= 4.092.  PMID: 23071744.

 

Zhao M. et al. 2011. Boty-like retrotransposons in the filamentous fungus Botrytis cinerea contain the additional antisense gene brtn. 417(2):248-52. Virology. IF= 3.351.  PMID: 21802103.

 

Li Y. et al. 2011. Generation of iPSCs from mouse fibroblasts with a single gene, Oct4, and small molecules. 21(1):196-204. Cell Res. IF= 8.19.  PMID: 20956998.

 

Xu PJ. et al. 2010. Apis mellifera has two isoforms of cytoplasmic HSP90. 19(4):593-7. Insect Mol Biol. IF= 2.529.  PMID: 20522122.

 

Lu B. et al. 2009. Expression and evolutionary divergence of the non-conventional olfactory receptor in four species of fig wasp associated with one species of fig. 9:43. BMC Evol Biol. IF= 3.521.  PMID: 19232102.