EasyScript First Strand cDNA Synthesis SuperMix

Product Overview

Description

EasyScript First-Strand cDNA Synthesis SuperMix

EasyScript^® First-Strand cDNA Synthesis SuperMix provides all the necessary components for cDNA synthesis from total RNA or mRNA. The cDNA is ef ficiently synthesized by EasyScript^® RT/RI Enzyme Mix and 2×ES Reaction Mix.

 

Cat. No.	AE301
Specification	AE301-02	50 rxns (20 μl per reaction)
	AE301-03	100 rxns (20 μl per reaction)
Storage:	at -20°C for one year
Advantages	Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNA synthesis.
	Anchored Oligo(dT)18 Primer for higher yield and more full length cDNA.
	cDNA up to 8 kb.
Applications	Multiple copy gene synthesis

 

Kit Contents

Component	AE301-02 (50 rxns)	AE301-03 (100 rxns)
EasyScript® RT/RI Enzyme Mix	50 μl	2×50 μl
2×ES Reaction mix.	500 μl	2×500 μl
Random Primer (0.1 μg/μl)	50 μl	100 μl
Anchored Oligo(dT)18 Primer (0.5 μg/μl)	50 μl	100 μl
RNase-free Water	500 μl	1ml

 

 

 

 

 

 

CITATIONS

Li H, et al. 2013. Decreased fructose-1, 6-bisphosphatase-2 expression promotes glycolysis and growth in gastric cancer cells. 12(1):110. Mol Cancer. IF=5.134. PMID: 24063558. 

 

Li Y, et al. 2013. Cotton GhMPK6a negatively regulates osmotic tolerance and bacterial infection in transgenic Nicotiana benthamiana, and plays a pivotal role in development. 280(20):5128-5144. FEBS J. IF=4.25. PMID: 23957843.

 

Wang X, et al. 2013. Microcystin (-LR) induced testicular cell apoptosis via up-regulating apoptosis-related genes in vivo. 60:309-17. Food Chem Toxicol. IF=3.01. PMID: 23891578.

 

Zhang Y, et al. 2013. A novel Omega-class glutathione S-transferase gene in Apis cerana cerana: molecular characterisation of GSTO2 and its protective effects in oxidative stress. 8(4):503-16. Cell Stress Chaperones. IF=2.484. PMID: 23382010.

 

Liu X, et al. 2013. MiR-18b inhibites TGF-ß1-induced differentiation of hair follicle stem cells into smooth muscle cells by targeting SMAD2. 438(3):551-6. Biochem Biophys Res Commun. IF=2.406. PMID: 23916701.

 

Peng M. 2010. Ectodomain shedding of Fcalpha receptor is mediated by ADAM10 and ADAM17. 130(1):83-91. Immunology. IF= 3.321.  PMID: 20059578.

  

Wang X. 2012. Microcystin (-LR) affects hormones level of male mice by damaging hypothalamic-pituitary system. 59(2):205-14. Toxicon. IF= 2.508.  PMID: 22178782.

 

Wu L. 2012. The effect of resveratrol on FoxO1 expression in kidneys of diabetic nephropathy rats. 39(9):9085-93. Mol Biol Rep. IF= 2.929.  PMID: 22733486.

 

Dai J. 2013. A novel DNA vaccine expressing the Ag85A-HA2 fusion protein provides protection against influenza A virus and Staphylococcus aureus. 10(1):40. Virol J. IF= 2.343.  PMID: 23369570.

 

Ying F. 2012. Comparative evaluation of nonylphenol isomers on steroidogenesis of rat Leydig Cells. 26(7):1114-21. Toxicol In Vitro. IF= 2.775.  PMID: 22771390.

  

Cao X. 2012. Inhibition on hepatitis B virus in vitro of lectin from Musca domestica pupa via the activation of NF-κB. 170(1-2):53-8. Virus Res. IF= 2.941.  PMID: 22940568.

 

Duan Q. 2012. ER stress negatively modulates the expression of the miR-199a/214 cluster to regulates tumor survival and progression in human hepatocellular cancer. 7(2):e31518. PLoS One. IF= 4.092.  PMID: 22359598.

 

Wu F. 2012. Identification of Major Active Ingredients Responsible for Burn Wound Healing of Centella asiatica Herbs. 2012:848093. Evid Based Complement Alternat Med. IF= 4.774.  PMID: 23346217.

 

Yu F. 2011. Molecular cloning and characterization of a thioredoxin peroxidase gene from Apis cerana cerana. 20(3):367-78. Insect Mol Biol. IF= 2.529.  PMID: 21382109.

 

Zhu FF. 2011. Generation of pancreatic insulin-producing cells from rhesus monkey induced pluripotent stem cells. 54(9):2325-36. Diabetologia. IF= 6.814.  PMID: 21755313.

 

Zhang Y. 2012. Expression of MMP-9 and WAVE3 in colorectal cancer and its relationship to clinicopathological features. 138(12):2035-44. J Cancer Res Clin Oncol. IF= 2.558.  PMID: 22806308.

 

Xu HY. 2013.12-Deoxyphorbol 13-palmitate mediated cell growth inhibition, G2-M cell cycle arrest and apoptosis in BGC823 cells. 700(1-3):13-22. Eur J Pharmacol. IF= 2.516.  PMID: 23220710.

 

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