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100bp Plus DNA Ladder
100bp Plus DNA Ladder is comprised of twelve linear double-stranded DNA bands. This ready-to-use product is premixed with 1× DNA loading buffer. Based on experiment requirement, 5 μl marker can be directly loaded for gel electrophoresis. It provides simple operation and produces clear image. For easy reference on agarose gels, the 500 bp band (100 ng/5 μl) is brighter than the other bands (50 ng/5 μl).
| Cat. No. | BM311 | |
| BM311-01 | 500 µl | |
| BM311-02 | 5×500 µl | |
| Storage: | at 4oC for six months; at -20oC for two years. | |
| Concentration: | 0.13 mg/ml | |
| Advantage | Suitable for accurate determination of the size of DNA fragment within the range of 100 bp-1500 bp | |
Composition | 100 bp, 200 bp, 300 bp, 400 bp, 500 bp, 600 bp, 700 bp, 800 bp, 900 bp, 1000 bp, 1200 bp, 1500 bp | |
| Storage Buffer | 10 mM Tris-HCl (pH 8.4), 10 mM EDTA, 0.02% bromophenol blue, 5% glycerol | |
| Instructions for Use | Load 100bp Plus DNA Ladder on the lane on a 2-3% agarose gel (load 1 μl marker for 1 mm lane, increase the loading volume for wider lane ). | |
| Suggested electrophoresis conditions: 2-3% agarose gel, voltage 4-10 v/cm. | ||
| Stained with EB or GelStain (GS101), visualize the bands under exposure to ultraviolet light. | ||
| Notes | Completely thaw and mix well before use. | |
| Please change the electrophoresis buffer in time and use freshly-prepared gel to avoid adverse effect on the result. | ||
CITATIONS
Han XY. et al. 2008. Molecular characterization of tree peony germplasm using sequence-related amplified polymorphism markers. 46(3-4):162-79. IF= 1.733. PMID: 18224436.



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