TransFast Taq DNA Polymerase PCR Enzyme

Product Overview

Description

TransFast ^® Taq DNA Polymerase

TransFast^®  Taq DNA polymerase is an engineered version of Thermus aquaticus DNA polymerase. The enzyme consists of a single polypeptide with a molecular weight of approximately 94 kDa.

TransFast ^®  Taq DNA polymerase has a 5′ to 3′ DNA polymerase activity and a 5′to 3′exonuclease activity lacking a 3′-5′ exonuclease activity.

 

Cat. No.	AP101
Specification	AP101-01:  500 units (dNTPS Free)
	AP101-02:  6 × 500 units (dNTPs Free)
	AP101-11:  500 units  (dNTPs 2.5mM)
	AP101-12:  6 × 500 units (dNTPs 2.5mM)
Concentration:	5 units/μl
Storage:	at -20°C for two years
Feathures	The extension rate is about 6 kb/min.
	Template-independent “A” can be generated at the 3′ end of the PCR product.  PCR product can be directly cloned  into pEASY®-T vectors.
	Amplification of genomic DNA fragment up to 4 kb.
Advantages	High efficiency amplification
	Rapid amplification
Application	Routine PCR
	Fast PCR
	Colony PCR
Notes	Recommended extension time can achieve optimal amplification performance, but increase the extension time may result in decreased amplification specificity.
	A final concentration of 2 mM MgSO4 is sufficient for most targets amplification. For some targets, more Mg2+ may be required; use the 100 mM MgSO4 stock to prepare a titration from 2 mM to 4 mM (final concentration) in 0.25 mM increments.
	0.5 μl (2.5 units) of TransFast® Taq DNA Polymerase is enough for a single 50 μl reaction. For better amplification, up to 1 μl (5 units)/reaction TransFast® Taq DNA Polymerase can be used.

 

Kit Contents

Component	AP101-01/11	AP101-02/12
TransFast ®  Taq DNA Polymerase	500 U × 1	500 U × 6
10× TransFast®  Taq Buffer(with Mg2+)	50 μl	250 μl
2.5 mM dNTPs	- / 900 μl ×1	- / 900 μl ×6
6×DNA Loading Buffer	20 μl	50 μl

 

 

 

 

 

 

 

 

 

 

 

Unit Definition: One unit (U) is defined as the amount of enzyme required to catalyze the incorporation of 10 nmol of dNTP into an acid-insoluble material in 30 minutes at 74^oC, with activated salmon sperm DNA used as template

 

Quality Control:  TransFast^® Taq DNA Polymerase has passed the following quality control assays: functional absence of double- and single-stranded endonuclease activity; >99% homogeneous measured by SDS-PAGE. Each batch of TransFast^® Taq DNA Polymerase has been assayed for amplification efficiency from as little as 10 ng of human genomic DNA.

 

CITATIONS

Bai N, et al. 2013. Yes-associated protein (YAP) increases chemosensitivity of hepatocellular carcinoma cells by modulation of p53. 14:511-520 Cancer Biol & Ther. IF=3.287. PMID: 23760493.

 

Wang Y. et al. 2012. The Arabidopsis APC4 subunit of the anaphase-promoting complex/cyclosome (APC/C) is critical for both female gametogenesis and embryogenesis. 69(2):227-40. Plant J. IF= 6.16.  PMID: 21910774.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

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