Easy Taq DNA Polymerase PCR Enzyme by TransGen Biotech

Product Overview

Description

EasyTaq DNA Polymerase

EasyTaq ^® DNA polymerase is purified from E. coli expressing a cloned DNA polymerase from Thermus aquaticus . The enzyme consists of a single polypeptide with a molecular weight of approximately 94 kDa. EasyTaq ^® DNA polymerase has  5′ to 3′ DNA polymerase activity and 5′to 3′exonuclease activity lacking 3′-5′exonuclease activity. EasyTaq ^® DNA polymerase is suitable for routine amplification. PCR products are unsuitable for PAGE.

 

Cat. No.	AP111
Specification	dNTPs-free	AP111-01	500 units
		AP111-02	6×500 units
		AP111-03	4×2500 units
	dNTPs(2.5 mM)	AP111-11	500 units
		AP111-12	6×500 units
		AP111-13	4×2500 units
Concentration:	5 units/μl
Storage:	at -20°C for two years
Description	The extension rate is about 1-2 kb/min.
	Template-independent “A” can be generated at the 3′ end of the PCR product. PCR products can be  directly cloned  into pEASY®-T vectors.
	Amplification of genomic DNA fragment up to 4 kb.
Advantages	High efficiency amplification
Application	Routine PCR
	Short fragment PCR

 

Unit Definition

One unit(U) is defined as the amount of enzyme required to catalyze the incorporation of 10 nmol of dNTP into an acid-insoluble material in 30 minutes at 74°c, with activated salmon sperm DNA used as template.

 

Quality Control

Easy Taq^® DNA polymerase has passed the following quality control assays: functional absence of double- and single-stranded endonuclease activity, >99% homogeneous measured by SDS-PAGE. Easy Taq^® DNA polymerase has been assayed for amplification efficiency from as little as 10 ng of human genomic DNA.

 

Notes

• A final concentration of 2 mM MgSO₄ is sufficient for most targets amplification. For some targets, more Mg²⁺ may be required; use the 100 mM MgSO₄ stock to prepare a titration from 2 mM to 4 mM (final concentration) in 0.25 mM increments.

• 0.5 μl (2.5 units) enzyme is enough for a single 50 μl reaction. For better amplification, up to 1 μl (5 units) enzyme can be used for a single reaction.

 

CITATIONS

He X-L, et al. 2013. Lack of Structural Variation but Extensive Length Polymorphisms and Heteroplasmic Length Variations in the Mitochondrial DNA Control Region of Highly Inbred Crested Ibis, Nipponia nippon. 8(6): e66324. PLos One. IF= 3.73. PMID: 23805212.

 

Pang Z, et al. 2013. Resistance to the Novel Fungicide Pyrimorph in Phytophthora capsici: risk assessment and detection of point mutations in CesA3 that confer resistance. 8(2): e56513. PLos One. IF=3.73. PMID: 23431382.

 

 

Xu W, et al. 2013. Transcriptome-wide identification and characterization of microRNAs from castor bean (Ricinus communis L.). 8(7):e69995. PLos One. IF=3.73. PMID: 23894571.

 

Li X, et al. 2013. Molecular diversity of arbuscular mycorrhizal fungi associated with two cooccurring perennial plant species on a Tibetan altitudinal gradient. 1-13. Mycorrhiza. IF=2.955. PMID: 23912811.

 

 

 

Li X, et al. 2013. Polymorphisms of anti-lipopolysaccharide factors in the swimming crab Portunus trituberculatus and their association with resistance/susceptibility to Vibrio alginolyticus. 34(6):1560-8. Fish Shellfish Immunol. IF=2.964. PMID: 23567857.

 

 

Lu Y. et al. 2013. Nonstructural proteins of Torque teno sus virus 2 from O2AUG: Preduction to experimental validation. Virus Res. IF=2.745. PMID: 24091363.

 

 

Zhang WY. et al. 2012. Highly parallel single-molecule amplification approach based on agarose droplet polymerase chain reaction for efficient and cost-effective aptamer selection. 84(1):350-5. Anal Chem. IF= 5.856.  PMID: 22103644.

 

Liu X. et al. 2011. Identification of novel variants of metadherin in breast cancer. 6(3):e17582. PLoS One. IF= 4.092.  PMID: 21408129.

 

Jiang X. et al. 2012. In vitro assembly of multiple DNA fragments using successive hybridization. 7(1):e30267. PLoS One. IF= 4.092.  PMID: 22291927.

 

Li Y. et al. 2011. A universal multiplex PCR strategy for 100-plex amplification using a hydrophobically patterned microarray. 11(21):3609-18. Lab Chip. IF= 5.67.  PMID: 21909519.

 

Zhu L. et al. 2012. In vitro selection of highly efficient G-quadruplex-based DNAzymes. 84(19):8383-90. Anal Chem. IF= 5.856.  PMID: 22954361.

 

Jefferies R. et al. 2009. Angiostrongylus vasorum from South America and Europe represent distinct lineages. 136(1):107-15. Parasitology. IF= 2.961.  PMID: 19126274.

 

Wang DW. et al. 2011. Identification of a Male-Specific Amplified Fragment Length Polymorphism (AFLP) and a Sequence Characterized Amplified Region (SCAR) Marker in Eucommia ulmoides Oliv. 12(1):857-64. Int J Mol Sci. IF= 2.598.  PMID: 21340018.

 

Qie F. et al. 2012. Extracting genomic DNA of foodstuff by polyamidoamine (PAMAM)-magnetite nanoparticles. 93:166-71. Talanta. IF= 3.794.  PMID: 22483894.

 

Wu F. et al. 2012. Identification of Major Active Ingredients Responsible for Burn Wound Healing of Centella asiatica Herbs. 2012:848093. Evid Based Complement Alternat Med. IF= 4.774.  PMID: 23346217.

  

Chen L. et al. 2012. Assessing the risk that Phytophthora melonis can develop a point mutation (V1109L) in CesA3 conferring resistance to carboxylic acid amide fungicides. 7(7):e42069. PLoS One. IF= 4.092.  PMID: 22848705.

 

Zheng T. et al. 2012. Development of a simvastatin selection marker for a hyperthermophilic acidophile, Sulfolobus islandicus. 78(2):568-74. Appl Environ Microbiol. IF= 3.829.  PMID: 22081574.

 

Zhang N. et al. 2011. BCL-2 (-938C > A) polymorphism is associated with breast cancer susceptibility. 12:48. BMC Med Genet. IF= 2.328.  PMID: 21457555.

 

Zhao HL. et al. 2012. Elastolytic mechanism of a novel M23 metalloprotease pseudoalterin from deep-sea Pseudoalteromonas sp. CF6-2: cleaving not only glycyl bonds in the hydrophobic regions but also peptide bonds in the hydrophilic regions involved in cross-linking. 287(47):39710-20. J Biol Chem. IF= 4.773.  PMID: 23012370.

 

Wu Y. et al. 2012. Molecular cloning and characterization of an endogenous digestive β-glucosidase from the midgut of the fungus-growing termite Macrotermes barneyi. 21(6):604-14. Insect Mol Biol. IF= 2.529.  PMID: 23126269.

 

Lu B. et al. 2009.Expression and evolutionary divergence of the non-conventional olfactory receptor in four species of fig wasp associated with one species of fig.9:43. BMC Evol Biol. IF= 3.521.  PMID: 19232102.