TransGen Biotech TransScript II Probe One Step qRT PCR SuperMix One step RT PCR Master Mix Nucleic Acid Test Kit RT PCR Test Kit

Product Overview

Description

Cat. No.  AQ321

Ordering Information:

AQ321-01	100 rxns (20 μl per reaction)
AQ321-02	400 rxns (20 μl per reaction)

 

Storage:  at -20°C for two years

Shipping: Dry ice (-70 ℃)

 

Description

TransScript^® II Probe One-Step qRT-PCR SuperMix is a one-step qRT-PCR kit with high sensitivity, high synthesis efficiency and high amplification efficiency. It contains all the necessary reagents for cDNA synthesis and qPCR except probe, total RNA/mRNA template and gene specific primers.

 

• High specificity, high sensitivity, high efficiency.

• Easy and rapid, reducing contamination.

• Compatibility with various real-time cyclers with passive reference dyes provided for different qPCR instruments.

  

Applications

• Multiple copy and low copy gene detection

• GC-rich templates or complex secondary structure RNA templates

• Viral RNA and trace RNA detection

 

Kit Contents

Component	AQ321-01	AQ321-02
TransScript® II One-Step RT/RI  Enzyme Mix	40 μl	160 μl
2×TransStart®Probe qPCR SuperMix	1 ml	4×1 ml
Passive Reference Dye (50×)	40 μl	160 μl
RNase-free Water	1 ml	4×1 ml

 

Reaction Components

Component	Volume	Final Concentration
RNA Template	Variable	as required
Forward GSP (10 μM)	0.4 μl	0.2 μM
Reverse GSP (10 μM)	0.4 μl	0.2 μM
Probe (10 µM)	0.4 μl	0.2 µM
2×TransStart® Probe qPCR SuperMix	10 μl	1×
TransScript® II One-Step RT/RI Enzyme Mix	0.4 μl	-
Passive Reference Dye (50×) (optional)	0.4 μl	1×
RNase-free Water	Variable	-
Total volume	20 μl	-

 

Notes

• Avoid any RNase contamination.

• High quality RNA template is recommended for use to ensure successful cDNA synthesis.

• This kit is only suitable for GSP, but unsuitable for first-strand cDNA synthesis using Oligo(dT) or Random Primer.