pEASY Blunt Simple Cloning Kit

Product Overview

Description

pEASY-Blunt Simple Cloning Kit

pEASY^® -Blunt Simple Cloning Vector eliminates the multi-cloning sites of pEASY^® -Blunt Cloning Vector. It is designed for cloning and sequencing Pfu-amplified PCR products.

Cat. No.	CB111
Sprcification	CB111-01:  20 rxns
	CB111-02:  60 rxns
Storage	Trans1-T1 Phage Resistant Chemically Competent Cells at -70oC for six months
	Others at -20oC for nine months
Description	5 minutes fast ligation of Pfu-amplified PCR products.
	Kanamycin and Ampicillin resistance genes for your choice of selection.
	Easy blue/white selection.
	SR primer and M13 forward primer for sequencing.
	T7 promoter for in vitro transcription.
	Trans1-T1 phage resistant chemically competent cell, high transformation efficiency (>109 cfu/μg pUC19 DNA) and fast growing.

 

 

Kit Contents

Components	CB111-01 (20 rxns)	CB111-02 (60 rxns)
pEASY®- Blunt Simple Cloning Vector (10 ng/μl)	20 μl	3×20 μl
Control Template (5 ng/μl)	5 μl	5 μl
Control Primers (10 μM)	5 μl	5 μl
M13 Forward Primer (10 μM)	50 μl	150 μl
M13 Reverse Primer (10 μM)	50 μl	150 μl
SR Primer (10 μM)	50 μl	150 μl
Trans1-T1 Phage Resistant Chemically Competent Cell	10×100 μl	30×100 μl

 

CITATIONS

Yu Z, et al. 2013. Phylogenetically diverse endozoic fungi in the South China Sea sponges and their potential in synthesizing bioactive natural products suggested by PKS gene and cytotoxic activity analysis. 58(1): 127-141. Fungal Divers. IF=5.319.    

 

Han M, et al. 2013. The ammonia oxidizing and denitrifying prokaryotes associated with sponges from different sea areas. 66(2):427-36. Microb Ecol. IF=3.277. PMID: 23435827.

 

Zhou K. et al. 2011. Phylogenetically diverse cultivable fungal community and polyketide synthase (PKS), non-ribosomal peptide synthase (NRPS) genes associated with the South China Sea sponges. 62(3):644-54. Microb Ecol. IF= 2.912.  PMID: 21519913.

 

Cheng F. et al. 2011. A novel frame-shift mutation of GLI3 causes non-syndromic and complex digital anomalies in a Chinese family. 412(11-12):1012-7. Clin Chim Acta. IF= 2.535.  PMID: 21320477.

 

Han M. et al. 2012. Bacterial and archaeal symbionts in the South China Sea sponge Phakellia fusca: community structure, relative abundance, and ammonia-oxidizing populations. 14(6):701-13. Mar Biotechnol (NY). IF= 3.43.  PMID: 22310803.

 

Shi Z. et al. 2012. Low Diversity Bacterial Community and the Trapping Activity of Metabolites from Cultivable Bacteria Species in the Female Reproductive System of the Oriental Fruit Fly, Bactrocera dorsalis Hendel (Diptera: Tephritidae). 13(5):6266-78. Mar Int J Mol Sci. IF= 2.598.  PMID: 22754363.

 

Zhang F. et al. 2012. Detection and quantification of cultured marine Alexandrium species by real-time PCR. 28(12):3255-60. World J Microbiol Biotechnol. IF= 1.532.  PMID: 22864601.

 

 

 

 

 

 

 

 

 

 

 

 

 

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