GelStain

Product Overview

Description

GelStain

GelStain is a sensitive, stable and safe staining reagent for DNA/RNA. GelStain uses the same wavelength as ethidium bromide (EB), and it is more sensitive than EB.

 

Cat. No.	GS101
	GS101-01	500 μl
Storage:	at 4oC in dark for one year
Concentration:	10000×
Characteristics	Non toxicity: GelStain is a specific form of oily macromolecules, which are incapable of entering cells via the cell membrane.
	High sensitivity: GelStain is suitable for different sizes of DNA.
	Exceptional stability: GelStain can be heated or microwaved.
	Signal to noise ratio: Strong fluorescent signal from samples, weak from background.
	Like EB, GelStain can be used before electrophoresis gel or after electrophoresis. No destaining is needed.
	No optical setting change: standard EB filter and SYBR filter can be used.

 

 

Staining Protocols

1. Post-Staining Protocol

• Run gels according to your standard protocol.

• Dilute GelStain 10,000× stock solution 3,300 fold to make a 3× staining solution in H₂O. Generally 50 ml staining solution is an adequate volume for one minigel. (e.g. add 15 μl GelStain 10000× stock reagent and 5 ml 1 M NaCl into 45 ml H₂O).

Note: including 0.1 M NaCl in the staining solution enhances sensitivity, but may promote dye precipitation if the gel stain is reused.

• Place the gel in a suitable container such as a polypropylene staining tray. Add a sufficient amount of the 3× staining solution to submerge the gel.

• Agitate the gel gently at room temperature for ~30 minutes.

Note: Optimal staining time may vary somewhat depending on the thickness of the gel and the percentage of agarose. For polyacrylamide gels containing 3.5-10% acrylamide, typical staining time is 30 minutes to 1 hour with gels of higher acrylamide content requiring longer staining time.

• Destaining is not required, but the gel can be washed in water to reduce background if necessary.

• Staining solution can be reused at least 2-3 times. Store staining solution at room temperature protected from light.

2. Precast Protocol for Agarose Gels

• Prepare molten agarose gel solution using your standard protocol.

Note: the precast protocol is not recommended for polyacrylamide gels. Polyacrylamide gels can be stained using the post-stain protocol.

• Dilute the GelStain 10,000× stock reagent into the molten agarose gel solution at 1:10,000 and mix thoroughly (e.g. add 1 μl GelStain stock reagent for per 10 ml agarose gel solution). GelStain can be added while the gel solution is still hot.

• Cast the gel and allow it to solidify.

• Load samples and run the gels using your standard protocol.

• Unused agarose containing GelStain can be remelted to cast more gels, but it may be necessary to add more dye for optimal signal. We do not recommend storing agarose containing GelStain in molten form (i.e., at 50^oC) for more than a few days. Precast gels containing GelStain can be stored at 4^oC for future use.

 

 

 

 

 

 

 

 

 

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