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Nitrogen cannot be absorbed by the plants owing to the strong triple bonds between the nitrogen atoms making it inert and therefore non assimable by plants.
Enzyme production: Azotobacter vinelandii produces enzymes such as ferredoxin, hydrogenase and nitrogenase
Nitrogen fixation needs a large amount of energy in the form of Adenosine Tri-Phosphate (ATP) and an anaerobic environment (no oxygen environment). Thus Azotobacter vinelandii develops a special defence mechanism against oxygen by intensifying its metabolism that reduces the oxygen concentration in its cells. A special protein sheath is present that protects the important nitrogenase enzyme. Homocitrate ions play some role in the processes of Biological Nitrogen Fixation (BNF).
Nitrogenase action: This is an important enzyme used to convert atmospheric nitrogen into ammonium / nitrate ions which get fixed in soil and can be absorbed by the plants. The basic type is molybdenum-iron nitrogenase.
Bio active compound synthesis: Azotobacter vinelandii synthesizes some biologically active substances including some phytohormones such as auxins thereby stimulating plant growth. They also facilitate the mobility of heavy metals in the soil and thus enhance bioremediation of soil from heavy metals, such as cadmium, mercury and lead.
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