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The Illumina NextSeq 500 Genetic Sequencer is a benchtop, compact DNA sequencer built with Illumina next-generation sequencing for higher coverage and fast sample processing. This genetic sequence runs one sample at a time, using a one-lane flowcell capable of 400 million reads per run. As a result, users get high multiplexing power while reducing overall cost per base. The Nextseq gives you the processing power and speed of a MiSeq but at a higher throughput.
The Illumina NextSeq 500 provides 1x75bp, 1x150bp, 2x75bp, and 2x150bp outputs. Mid-output sequencing kits also provide up to a maximum of about 130 million reads per run. Run times for this sequencer are rapid, with times between 11 hours (75bp single read) to 26 hours (300bp paired-end read). By using different kits, users can tailor the runs to their needs. These kits are optimized for low input, rapid workflows, and bias-free coverage.
The Nextseq 500 features whole genome (re)sequencing, comprehensive detection of mutations and SNPs in the entire genome, RNA expression profiling, ChIP-sequencing, DNA methylation profiling, and more at the touch of a button. Data is easily accessible for analysis and mining, to come back to again and again as discoveries are made. The user-friendly software and information tools make analysis and interpretation accessible for a deeper understanding of biology.
Specification | Details |
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Required Input | 100 ng–1 μg with TruSeq Library Prep Kits |
Data Quality | > 75% higher than Q30 at 2 × 150 bp |
High Output Flow Cell | |
- Sample | 1 x 30× human genome |
- Read Length | 2 × 150 bp |
- Output | 100–120 Gb |
- Run Time | 29 hours |
- Single Reads | Up to 400 M |
- Read Length | 2 × 75 bp |
- Output | 50-60 Gb |
- Run Time | 18 hours |
- Paired-End Reads | Up to 800 M |
- Read Length | 1 × 75 bp |
- Output | 25-30 Gb |
- Run Time | 11 hours |
Mid Output Flow Cell (130 M) | |
- Number of Samples | Up to 24 small genomes |
- Read Length | 2 × 150 bp |
- Output | 32-39 Gb |
- Run Time | 26 hours |
- Single Reads | Up to 130 M |
- Paired-End Reads | Up to 260 M |
- Read Length | 2 × 75 bp |
- Output | 16-19 Gb |
- Run Time | 15 hours |
Dimensions | 21 × 25 × 24 |
Weight | 183 lbs |
Power | 100-240 V, 50-60 Hz, 600 W |
INCLUDED
Illumina NextSeq 500 Genetic Sequencer | SY-415-1001
Built-in Computer Running NextSeq Control Software
Touch Screen Controller
Keyboard & Mouse
Sample Racks
Buffer Cartridge
User Manual
120V Power Cord (6A)
30-Day Warranty
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