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Цена:70,00 $ - 80,00 $*

Количество:

  • В избранное

Описание и отзывы

Характеристики


AO Stain Kit



Product Description


 Cat:CA1142




Size:100T


Storage:4℃, avoid light, valid for 6 months. 


Kit Components






















Reagent



100T



Storage



Reagent(A):AO Stain Solution



500µl



4℃, avoid light



Reagent(B):AO Stain Buffer(10×)



10ml



4℃




Introduction


AO belongs to tricyclic heteroaromatic fuel, which can mark DNA and RNA. It belongs to heterochromic fluorescent dye. The dye has membrane permeability and can penetrate cell membrane to dye nuclear DNA and RNA. Therefore, AO is often used to detect DNA and RNA in cells. The binding modes of AO and nucleic acid are as follows: 1. Insertion binding. AO is embedded between the base pairs of nucleic acid double chain. This binding mode is mainly the combination of AO and DNA. Its fluorescence emission peak is 530nm, which is green fluorescence after excitation. 2. Electrostatic attraction. The positive charge AO and the single chain nucleic acid phosphate (with negative charge) produce electrostatic attraction binding, which is mainly the combination of AO and RNA. The fluorescence emission peak is 640nm which is red fluorescence after excitation. A small amount of binding will show orange or orange fluorescence. As a result, AO chimeras appear green in double stranded DNA molecules and emit orange or orange red fluorescence when combined with DNA single strand or RNA.


The AO Stain Kit is mainly composed of AO Stain Solution and AO Stain Buffer, which are often used to detect apoptosis. After staining, AO could make the nucleus show green or yellow green uniform fluorescence through the normal cell membrane, while in apoptotic cells, the chromatin is condensed or broken into fragments of different sizes, forming apoptotic bodies. AO can make them stained with dense and concentrated yellow green fluorescence or yellow green fragment particles, while yellow fluorescence of necrotic cells weakens or even disappears.AO staining is often combined with EB staining. EB staining only produces orange fluorescence in dead cells, which can distinguish normal cells, apoptotic cells and necrotic cells.



Packaging & Shipping


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Our Services


1. Sample require: we can provide sample.


    More details, welcome your contacting.


 


2. Quick delivery, usually within 1 to 3 days after your payment, within 10 days with mass quantity.


 


3. Fast and safe Shipping methods, such as DHL, FEDEX, TNT, UPS, EMS.


 


4. Various and convenient payment methods available, such as T/T, Western Union.


 


5. We insist: Customer first. High quality guarantee with best service for each customers.





Company Information


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Founded in 2004, Beijing Solarbio Science & Technology Co., Ltd. is a national high-tech enterprise, committed to providing high quality products and services for our loyal customers. Our products and services are mainly applied in molecular biology, cell biology, immunology and biomedical fields.


 


Solarbio has 30,00 square meters of research and production center, 2400 square meters of storage base and equipped with advanced logistics system. After 17 years of technological accumulation, we established an independent platform for immunology research and development. The platform include peptide design and synthesis, recombinant protein, antibody production and purification, primary antibody customization and related labeling and detection services, ELISA kit customization and other immunological product developments. Our mainly immunology products are antibodies and ELISA kits.


 


Now we can supply more than 10,000 primary antibodies, 300 secondary antibodies. With annual production capacity of 2000 antibodies we can provide more superior antibodies for customers. Solarbio technical team have rich experience in peptide design and recombinant protein expression, which enhanced the reliability of the antibodies. Solarbio have exceed 800 ELISA kits, the kits have high stability, good specificity and reliable quality. The detection species mainly include human, rat, mouse, bovine, swine and monkey. Besides these, we keep releasing new products, such as Cell separation medium, Staining kit, Biochemical assay kit, Reference materials, Cell signaling compounds. Through strict quality control, all of Solarbio products could ensured the authenticity and preciseness of the customers' experimental results.





FAQ


1.Q: How about your samples?


    A: Depends on your quantity.


 


2.Q: What is your delivery time?


    A: Within 7 days after payment.


 


3.Q: How about your payment methods?


    A: T/T, Western Union, Also the payment could via alibaba.


 


4.Q: Are your manufacture?


    A: Yeah, we are. Our company is located in Beijing. You are welcome to visit our company.


 


5.Q: Which shipping methods do you have?


    A: Shipping by International Express, such as DHL, FEDEX, TNT, UPS, EMS.





Contact information


Tina Zhao


Serve for you with all sincerity.


Beijing Solarbio Science & Technology Co., Ltd.


Third Floor, 85A, Lian dong U valley, Tongzhou Dist. Beijing, China.


Biochemical reagents; Stain; Elisa Kit; Antibody; Molecular biology; Cell culture medium; PCR reagents;DNA/RNA extract;  Small Molecule/Inhibitor; Standard...


 


 


 




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