Набор для обнаружения апоптоза TMR Red Tunel Cell

The breakage of chromosomal DNA in apoptosis is a gradual process. Chromosomal DNA is first degraded into large fragments of 50-300 kb under the action of endogenous nucleases, and then about 30% of chromosomal DNA is under the action of Ca2+ and Mg2+-dependent endonucleases. They are randomly cut to form 180-200 bp nucleosomal DNA polymer. Therefore, in the late stage of apoptosis, DNA will be degraded into 180-200 bp fragments, and a large number of 3'-OH ends will be exposed on the broken genomic DNA. Terminal Deoxynucleotidyl Transferase (TdT) is a template-independent DNA polymerase that can catalyze the binding of deoxynucleotides to the 3'-OH ends of broken DNA molecules. Therefore, TUNEL (TdT mediated dUTP Nick End Labeling) cell apoptosis detection kit can be used to detect the nuclear DNA breakage of tissue cells in the late stage of apoptosis. The principle is that under the action of TdT enzyme, tetramethyl-Rhodamine-dUTP (Tetramethyl-Rhodamine-5-dUTP, TMR-5-dUTP) is incorporated into the 3´-OH end exposed when genomic DNA is broken, so that it can be Detect with a fluorescence microscope or flow cytometer (TMR excitation 520-560 nm, emission 570-620 nm). This kit has a wide range of applications and is suitable for the detection of cell apoptosis in paraffin tissue sections, frozen tissue sections, cell slides, cell smears, etc.

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This product is for scientific research purposes only, not for clinical diagnosis!