Комплект ДНК салфеток SolPure D3312 экстракция из тканей

Сохранить в закладки 1600342016560:


Цена:27,00 $ - 45,00 $*

Количество:

  • В избранное

Описание и отзывы

Характеристики



Overview


Product Name: SolPure Tissue DNA Kit
Product Use Case: Isolation total DNA from Tissue and cells using economic Salt out method
Packaging Size: 1g/box; 5g/box; 50g/box
Storage:Room temperature
Shelf Life: 18 months


Order Information


Catalog #
Product Name
Packaging Size
List Price (FOB)
D331201
SolPure Tissue DNA Kit
1g/box
45 USD
D331202
SolPure Tissue DNA Kit
5g/box
150 USD
D331203
SolPure Tissue DNA Kit
50g/box
1350 USD


Note: 1g/box, 5g/box, 10g/box are for the largest amount of sample that the kit can deal with one time.


Introduction


SolPure DNA Kits use an improved salting-out purification method to provide a safe and economical solution for the extraction of genomic DNA from biological samples such as blood, tissue, and cultured cells. There is no need to use toxic phenol chloroform, or any expensive reagents. It is currently the most economical kit for nucleic acid extraction.

The kit has little restriction on the amount of samples, and can flexibly adjust various amounts of samples. The obtained DNA can be directly used for PCR, restriction digestion, Southern hybridization and other experiments.



Procedure


Cells are lysed with an anionic detergent in the presence of a DNA stabilizer. The DNA stabilizer limits the activity of intracellular DNases and also DNases found elsewhere in the environment. RNA is then removed by treatment with an RNA digesting enzyme. Other contaminants, such as proteins, are removed by salt precipitation. Finally, the genomic DNA is recovered by precipitation with alcohol and dissolved in Buffer TE. Purified DNA typically has an A260/A280 ratio between 1.7 and 1.9, and is up to 200 kb in size. The DNA can be safely stored at 2–8°C, –20°C, or –80°C.


Specifications


Main function
Isolation total DNA from Tissue and cells using economic Salt out method
Purified products
genomic DNA or mitochondrial DNA
Applications
PCR, restriction enzyme digestion, Southern hybridization, etc.
Operation Method
Salting out method
Sample type
tissue or cells
Sample Volume
unlimited
Minimum Elution Volume
100 ul
Extraction time
Varies with different sample volume
Operation
manual


Kit Contents




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