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Porcine circovirus (PCV) is a common virus of pigs found throughout the world. In the late 1990s, a novel circovirus emerged in North American swine that appeared to be genetically distinct from the prototype PCV (now referred to as PCV Type 1) and was termed PCV Type 2 (PCV2). PCV2 (and PMWS) has become widely distributed in most developed swine industries around the world. |
The PCV2 Ab Rapid Test Kit use chromatographic immunoassay for the qualitative detection of PCV2 antibody in blood. Sample to be tested is loaded to the sample pad, and then capillary flow along on the test strip. The detection antigen is coupled with colloidal gold as conjugate will mix with the sample fluid. Where PCV2 antibody is present, a complex is formed by PCV2 antibody and colloidal gold labelled antigen. The labelled antigen–antibody complex is then bound by a second ‘capture-antibody’ that recognizes the complex, and which is immobilized as T line on the test strip. A positive result therefore generates a visible wine-red line of antigen–antibody complex. A wine-red C line will appear to confirm the test is operated correctly.
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