Эрлихия анаплазма бабезия гибсони комбинированный тест антител (EHR/ANA/BAB)

Описание и отзывы

Характеристики

Introduction:

Ehrlichia – Anaplasma – Babesia gibsoni Antibody Combo Test

1. INTENDED USE

The Canivet EHR-ANA-BAB Ab Combo Test is a lateral flow immunochromatographic assay for the qualitative detection of Ehrlichia canis antibodies (E.canis Ab), Anaplasma spp. antibodies and Babesia gibsoni antibodies in dog’s serum specimen.

Assay Time:  5-10 minutes

2. PRINCIPLE

The Canivet EHR-ANA-BAB Ab Combo Test is based on sandwich method lateral flow immunochromatographic assay. The test card has a testing window for the observation of assay running and result reading. The testing window has an invisible T (test) zone and a C (control) zone before running the assay. When the treated sample was applied into each sample hole on the device, the liquid will laterally flow through the surface of the test strip and react with the pre-coated Ehrlichia, Anaplasma and Babesia recombinant antigens respectively. If there are Ehrlichia, Anaplasma and Babesia gibsoni antibodies in the specimen, a visible T line will appear. The C line should always appear after a sample is applied, which indicates a valid result. By this means, the device can accurately indicate the presence of Ehrlichia, Anaplasma and Babesia gibsoni antibodies in the specimen.

3. REAGENTS AND MATERIALS

- 10 test pouches, with cards and disposable droppers

- 10 vials of assay buffer   

- 1 package insert

4. STORAGE AND STABILITY

The kit can be stored at room temperature (2-30°C).

The test kit is stable through the expiration date marked on the package label.

DO NOT FREEZE.

Do not store the test kit in direct sunlight.

      5. SPECIMEN PREPARATION AND STORAGE

1. Specimen should be obtained and treated as below. Serum or plasma: collect the whole blood for the patient dog, centrifuge it to get the plasma, or place the whole blood into a tube which contains anticoagulants to get serum.

2.  All specimen should be tested immediately. If not for testing right now, they should be stored at 2-8℃.

6. TEST PROCEDURE

- Allow all materials, including specimen and test device, recover to 15-25℃ before running the assay.

- Take out the test card from the foil pouch and place it horizontally.

- Collect 20μL of the prepared specimen into a vial of assay buffer and mix well. Then drop 3 drops (approx. 120μL) of the diluted sample into the sample hole “S” of the test card.

Interpret the result in 5-10 minutes. Result after 10 minutes is considered as invalid.

6. INTERPRETATION OF RESULTS

- Positive (+): The presence of both “C” line and zone “T” line, no matter T line is clear or vague.

- Negative (-): Only clear C line appear. No T line.

- Invalid: No colored line appears in C zone. No matter if T line appears.

7. PRECAUTIONS

- All reagents must be at room temperature before running the assay.

- Do not remove test cassette from its pouch until immediately before use.

- Do not use the test beyond its expiration date.

- The components in this kit have been quality control tested as standard batch unit. Do not mix components from different lot numbers.

- All specimens are of potential infection. It must be strictly treated according to the rules and regulations by local states.

8. LIMITATION

Canivet EHR-ANA-BAB Ab Combo Test is designed for in vitro veterinary diagnosis use only.

All result should be considered with other clinical information available with veterinarian.

It is suggested to apply a further confirmative method such as PCR when positive result was observed.

Our Service:

Delivery:  DHL OR FEDEX

Our Company:

Shanghai S&C BIOTECH is a professional bio-tech products manufacturer,and a Specialist in the development, manufacture, and marketing of veterinary point of care diagnostic instruments and consumables as well as animal disease diagnostic detection kits. Our aim is to supply qualified products and services to our customers for better health care .Since the establishment of our company, we have been servicing hundreds of international customers,we are working every day to better understand and address the real-world challenges faced by those who raise and care for animals in ways they find truly relevant.