LSY 10049 медовые антибиотики хлортетракилин ELISA тестовый набор для молока меда мяса обнаружения безопасности кормов

Описание и отзывы

Характеристики

Green Spring Chlortetracyline ELISA Test Kit  

Catalog No. LSY-10049

1. Principle

This test kit is based on the competitive enzyme immunoassay for the detection of Chlortetracyline in the sample.The coupling antigens are pre-coated on the micro-well stripes. The Chlortetracyline in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti- Chlortetracyline antibody. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Chlortetracyline in it. This value is compared to the standard curve and the Chlortetracyline concentration is subsequently obtained.

2. Technical specifications

Sensitivity: 0.2 ppb

Incubation Temperature: 25℃

Incubation Time: 30min—15min

Detection limit:

Tissue(method 1).................................................................................................. about 3ppb

Tissue(method 2), Egg, Milk (method 2)......................................................... about 10ppb

Honey, Milk (method 1)........................................................................................ about 5ppb

Serum...................................................................................................................... about 2ppb

Feed...................................................................................................................... about 20ppb

Note: ppb=ng/mLor ng/g

Cross-reaction rate:

Chlortetracycline............................................................................................................. 120%

Tetracycline...................................................................................................................... 100%

Oxytetracyline.................................................................................................................... 50%

Doxycycline ...................................................................................................................... 20%

Recovery rate:  90±30%

3. Components

1)   Micro-well strips: 12 strips with 8 removable wells each

2)   10× concentrated standard solution: 0 ppb, 2ppb, 6ppb, 18ppb, 54ppb (0.5ml/bottle)

3)   Antibody working solution (7 mL)

4)   Enzyme conjugate (7 mL)

5)   Substrate A (7 mL)

6)   Substrate B (7 mL)

7)   Stop solution (7 mL)

8)   20× concentrated washing buffer (30 mL)

9)   10× concentrated redissolving solution (20 mL) 

Our Honey Antibiotics test kits

We are Shenzhen Lvshiyuan Biotechnology Co.,Ltd (www.lsybt.com), professional manufacturer and exporterof the most advanced Food Safety ELISA test kit(e.g. Nitrofuran (AMOZ, AOZ, AHD, SEM) ELISA kit, Clenbuterol ,Ractopamine ELISA kit), Animal Disease Diagnostic kits(e.g. Swine FMD lgG Distinguish kit,PRRS,PRV ELISA kit, NDV,IB,IBD ELISA kit),small molecule antigen and antibodies, gene recombination antigen and antibodies, etc.

Our advantages at the Diagnostic kits/ ELISA test kits field are as following:

* ISO9001&ISO13485 certificated company

* Own a high-quality R&D team with many years of experience on bio-diagnostic field.

* Professional manufacturer of ELISA test kits, offer OEM service.

*At present, our products have been exported to over 100 countries, such as United States, Belgium, France, Korea, Germany, Singapore etc.

*Delivery time: 1-3 days after confirmation by FedEx,DHL or other cheaper ways.

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