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Характеристики

Green Spring Benzyl penicillin ELISA detection kit

Catalog No.: LSY-10019

1.Principle

This test kit is based on the competitive enzyme immunoassay for the detection of Benzyl penicillin in samples. The antigens conjugated Benzyl penicillin is pre-coated on the micro-well stripes, Benzyl penicillin in the sample and the conjugated antigens pre-coated on the micro-well stripes compete for the anti-Benzyl penicillin antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with Benzyl penicillin concentration in the sample. This value is compared to the standard curve and concentration of Benzyl penicillin in the sample is subsequently obtained.

2. Technical specifications

Sensitivity: 0.1 ppb

Incubator temperature:  25℃

Incubator time:  30min~30min~15min

Detection limit:

Tissue, honey, milk ......................................... 2 ppb

Because of some interference, quantification limit of honey, milk sample is 4 ppb

Recovery rate:

Tissue .............................................................75±19%

Honey, milk .....................................................70±17%

Cross-reaction rate:

Benzyl penicillin ..............................................100%

Ampicillin .........................................................0.8%

Cloxacillin ........................................................0.2%

Dicloxacillin ......................................................0.1%

Amoxicillin ........................................................0.1%

Ceftiofu .............................................................0.1%

3. Components

1) Micro-well strips: 12 strips with 8 removable wells each

2) 6× standard solution (1 mL each): 0 ppb, 0.1 ppb, 0.3 ppb, 0.9 ppb, 2.7 ppb and 8.1 ppb

3) Enzyme conjugate (12 mL) red cap

4) Antibody working solution (7 mL) blue cap

5) Substrate A solution (7 mL) white cap

6) Substrate B solution (7 mL) black cap

7) Stop solution (7 mL) yellow cap

8) 20× concentrated washing buffer (40 mL) white cap

9) 5× concentrated redissolving solution (50 mL) transparent cap

4. Materials required but not provided

1) Equipments: microplate reader, printer, homogeniser, nitrogen-drying device, vortex, centrifuge, measuring pipets, balance (a reciprocal sensibility of 0.01 g)

2) Micropipettors: single-channel 20~200 µL and 100~1000 µL, and multi-channel 30~300 µL ;

3) Reagents: NaOH, Acetonitrile(CH3CN), N-Hexane, deionized water, Na2Fe(CN)5·NO·2H2O, ZnSO4·7H2O, HCI(36%), ZnSO4

Our Food Safety ELISA kits(Antibiotic Residues)

ISO9001:2015 Certificate

We are Shenzhen Lvshiyuan Biotechnology Co.,Ltd (www.lsybt.com), professional manufacturer and exporterof the most advanced Food Safety ELISA test kit(e.g. Nitrofuran (AMOZ, AOZ, AHD, SEM) ELISA kit, Clenbuterol ,Ractopamine ELISA kit), Animal Disease Diagnostic kits(e.g. Swine FMD lgG Distinguish kit,PRRS,PRV ELISA kit, NDV,IB,IBD ELISA kit),small molecule antigen and antibodies, gene recombination antigen and antibodies, etc.

Our advantages at the Diagnostic kits/ ELISA test kits field are as following:

* ISO9001&ISO13485 certificated company

* Own a high-quality R&D team with many years of experience on bio-diagnostic field.

* Professional manufacturer of ELISA test kits, offer OEM service.

*At present, our products have been exported to over 40 countries, such as United States, Belgium, France, Korea, Germany, Singapore etc.

*Delivery time: 1-3 days after confirmation by FedEx,EMS or other cheaper ways.