Экстракция и обнаружение хинолинового иммуноаффинного остатка ветеринарного препарата IAC314 из корма других образцов

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IAC314 Veterinary drug residue quinoline immunoaffinity column

A simple and efficient extraction and purification procedure for Olaquindox was developed by means of the immunoaffinity column (IAC-OLA) as a cleanup tool.Olaquindox content in Feeds are cleaned up by IAC and determined by HPLC or LC-MS. It is a fast,simple, safe and highly accurate method for quantitatively measuring Olaquindox.



Principle
Samples are prepared by mixing with an extraction solution, blending and filtering. The extract is then applied to the Olaquindox immunoaffinity column bound with specific antibodies to Olaquindox .At this stage, the Olaquindox binds to the antibody on the column. The column is then washed with water to remove the impurities in immunoaffinity column. By passing acetone through the column, the Olaquindox is removed from the antibody. This acetone solution can then be injected into HPLC or LC-MS/MS system.








Storage condition
The affinity column is stored at 2-8℃ and must not be frozen, with a shelf life of 18 months. Recommended for use at room temperature (18-30℃).


Application field
Food, Chemical, biological industry, pharmaceutical, general


Main use
It is widely used in the extraction of feed and other samples.




Operation procedure



Matters needing attention
1、Do not arbitrarily change the operation steps in the operating instructions. If you need to change them, contact the technical department of the company to confirm whether the changes are reasonable.
2、in the operation steps, the sample through the column, washing, elution, be sure to control the flow rate, not too fast, otherwise it will make the test result low.
3、if the standard product directly through the column to verify the recovery rate, be sure to ensure that the concentration of methanol in the standard column liquid does not exceed 10%, otherwise it will affect the adsorption capacity of the column.
4、the glass instruments used in the test, etc., must be cleaned, especially the instruments treated with hypochlorous acid, otherwise the test results will be low.


Specifications


Format
3ml
Specificity
Olaquindox
Column capacity
≥500ng
Storage Conditions
2 - 8 °C
Shelf Life
18 Months
Recovery
≥80%


Method Information


Extraction
purified water
Lamp
deuterium or mercury lamp
Detector
260 nm
Column
Cloversil -C18,4.6×250mm(5um)
Mobile Phase
methanol: water (20:80) isocratic degassed
Injection Volume
50-200 µl
Flow rate
1.0ml/min


HPLC chromatogram of Olaquindox 100ng/mL



Equipment and reagents to be prepared


C/N
item
ZJ0612
CLOVER multi-position pump flow operator(equipped with 1 air pump, 6 10mL syringes)
20202
High speed homogenizer, speed up to 18,000 r/min ~22,000 r/min
31955C
clover Microfiber Filter Paper (1.5um, 100 sheets/box)
31240C
clover folding grooved filter paper (100 sheets/box), or medium speed qualitative filter paper
36010C
Disposable plastic beaker (25/ pack)
34000C
Disposable test tube (250 PCS/pack)
36020C
Plastic funnel (10 PCS/pack)
35016
Chromatographic pure grade methanol (4 L/bottle)
C546150-U
Cloversil C18 Reversed Phase Column 4.6*150mm (5um)
C546250-U
Cloversil C18 Reversed Phase Column 4.6*250mm (5um)


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GOHI Technology sell all the products and services in the international market as a representative of Beijing ClovertechLimited Company who began to focus on the development of a series of food safety testing products such as mycotoxins, veterinary drug residues, pesticide residues, irradiated food, heavy metals, water density, etc from 2002. Over the years, we have reached cooperation with quality inspection, grain and oil, agriculture and other departments. We have obtained many patent results and participated in the formulation of many Chinese national standards and CIQ industry standards.

The factory located in Beijing. We produce and sell various testing instruments and Elisa Kits of practical food, agricultural and sideline products, feed; analytical instruments, biological and chemical reagents, etc.; the testing technology and equipment provided by us are in line with the standards confirmed by AOAC, EPA, and FDA. These methods have the characteristics of: accurate, fast, simple and safe.

The service methods we provide: advanced method research, technical difficulties discussion, solution suggestions, test operation training, scientific research process participation, and research results promotion. The company presents not only products and technologies, but also friendship and knowledge, joy and success that we share with friends!


A vision of our factory
The exterior of our factory building


Our group took a family photo
Exhibition group photo


Introduce to users in the exhibition
Office building interior display


Invention patent certificate
Quality Management System Certificate ISO9001
Science and technology award certificate




Q1.Do you have MOQ?
Depends on different ideas, Can be negotiated.The larger the quantity is, the competitive the unit price will be.

Q2.Should the customer pay the delivery fee,How much is it?
For the delivery fee,many samples are be requested to be sent,so we must get delivery fee.
If you tell me to use the appointed Express,you will give me your express account or you will pay
according to the Express.
If you do not request,I will choose a cheap one in China.

Q3.How about the after sale service?
1)We will always keep the quality the same as the buyer's samples and if there is something with the quality,
we will make compensation for our customers.
2)We will suggest our packing and take charge in our packing,we will keep the goods safe in the delivery.
3)We will trace the goods from the production to selling,we will solve the problems in the selling for our customers.

Q4.When can I get a price?
We usually quote within 24 hours after we get your inquiry.

Q5: Are you a trade company or manufacturer?
We are a professional manufacturer with our own factory.




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