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Universal TaqMan multiplex qPCR master mix -11211ES
Universal Blue qPCR Master Mix (Probe Based) is a pre-solution for 2×real-time quantitative PCR amplification characterized by high sensitivity and specificity, is blue in color, and has the effect of sample addition
This product is a 2× Mix pre-mixed reagent that enables up to four fluorescent quantitative PCR reactions in a single reaction well. This product contains the genetically modified antibody method to hot-start Taq enzyme, greatly improving the amplification sensitivity and specificity. At the same time, this product has deeply optimized the multi-reaction buffer, which can improve the amplification efficiency of the reaction and promote the effective amplification of low-concentration templates. This product can be used for genotyping and multiplex quantitative analysis.
Features
· Strong tolerance of blood impurities
· High sensitivity
· Super storage stability
Applications
· Pathogen detection
· Copy number analysis
· SNP genotyping
Hot Start | Built-in hot start |
Detection method | Primer-probe detection |
PCR method | qPCR |
Polymerase | Taq DNA polymerase |
Type of sample | DNA |
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No. | Name | 11211ES03 100T | 11211ES08 500T | 11211ES20 2,000T | 11211ES61 10,000T |
11211 | Universal Multiplex qPCR Master Mix (Probe Based) | 1 mL | 5×1 mL | 20 mL | 100 mL |
Components | Volume (μL) | Volume (μL) | Final Concentration |
2× Universal TaqMan multiplex qPCR master mix | 10 | 12.5 | 1× |
Primer mix (10 μmol/L) | x | x | 0.1-0.5 μmol/L |
Probe mix (10 μmol/L) | x | x | 50-250 nmol/L |
Rox reference dye | 0.4 | 0.4 | 1× |
Template DNA/cDNA | 1-10 | 1-10 | - |
ddH2O | up to 20 | up to 25 | - |
Rox reference dye is optional
CYCLE STEP | TEMPERATURE | TIME | CYCLES |
Initial Denaturation | 95°C | 5 mins | 1 |
Denaturation | 95°C | 15 secs |
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The product is shipped with dry ice and can be stored at -15℃ ~ -25℃ for 2 year.
Figure 1 High stability: 37℃ thermal acceleration stability
The red line: 37℃ thermal acceleration 0 day; The yellow line: 37℃ thermal acceleration 7 days; The blue line: 37℃ thermal acceleration 14 days
Figure 2 High stability: freeze-thaw stability
The red line: freeze thaw o time; The yellow line: freeze thaw 50 times; The blue line: NTC
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Found in 2005,Saturn Medical is a subsidiary of Saturn Group.Our company is committed to the R&D,production and sales of medical devices. We provide OEM and ODM service.
Our business is covered more than 108 countries,In China, we are working with over 1,000 hospitals and clinics. All products have been approved by CE certification.
Our main product including diagnostic reagents,Semi-manufactured reagent material like molecular enzyme,vet medical device,etc..
Our mission is “to provide vehospitals all over the word with safe,stable and economical medical and lab device”.
Packing
Packed in carton box.
Delivery
Goods could be shipped by express,by air or by sea,depends on customers‘ requirements.
Lead time
Our normal lead time is within 7-14 days after payment.
Every year,we will attend the exhibitions all over the world,
Sincerely invite you to visit our booth.
1.What's your advantages ?
1) We only focus on medical field,more than 10 years in medical experience.
2) Our business covers more than 90 countries.
3) We offer you competitive price,our aim is to achieve win-win.
4) 24 hours online service for you.
5) On time delivery
2.What certifications your products have.
CE ,ISO,FSC
3. Do you accept OEM service.
Yes.
4.What is your lead time?
Order quantity <5 sets: about 7-14 days after receipt of 100% T/T payment.
Mass order:about 15 days after receipt of 50% T/T deposit payment.
5. How is your warranty?
1-2 years depends on different machines. Lifetime maintain.
6.What about your after-sale service?
1) All products will be strictly Quality Checked and be well packed before shipping.
2)We have professional after sales service team,experienced engineers to help you solve any problem you meet
7. Can you offer the technical support, online or site training ?
We will offer technical support like operating manual, operating videos For some machines, we can offer you oversea training.
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