Морских животных ткани геномной набора извлечения ДНК комплект для очистки N1171/N1172

Описание и отзывы

Характеристики

   Marine Animal Tissue Genomic DNA Ectraction Kit

                     For research use only

Kit Content

Description

Marine Animal Tissue Genomic DNA Purification Kit provides a simple and rapid method for high quality genomic DNA purification from tissues(either fresh or frozen at -70°C until use) of marine animal, such as fish, shrimp, shellfish or crab. The Genomic DNA system uses the silica-gel-membrane technology for simple and fast isolation of Genomic DNA without phenol/chloroform. Homogenization is not necessary since tissues are directly lysed by Proteinase K. The buffer system is optimized to allow selective binding of DNA to the silica-gel membrane. The simple centrifugation protocol completely removes contaminants such as proteins, divalent cations, and secondary metabolites. Pure DNA is then eluted in water or low-salt buffer, ready to use. The typical yield of genomic DNA is 3-35 µg from 10 mg of tissue. The purified high molecular weight genomic DNA is suitable for direct use in all common molecular biology applications: PCR, restriction digestion, cloning, DNA sequencing and Southern blot analysis.

Applications

Purified DNA is free from contaminants and enzyme inhibitors, and typically has A₂₆₀/A₂₈₀ ratios between 1.7 and 1.9, and is suitable for applications such as:

• Restriction digestion
• PCR
• Labeling
• Library construction

Feature. 

• Fast - procedure takes only 30 min.
• High Efficient – 3-35 µg of genomic DNA from 10 mg of tissue or 1 x 10⁶-10⁷ cultrue cells.
• Safe - no phenol/cholroform extraction step.
• High purity - Purified DNA is ready for downstream application such as PCR, restriction digestion.

Store

store Protein K at -20°C, other reagents can be store at room temperature for up to 1 year. Any precipitate in the Solution DS and solution MS can be re-dissolved by incubating at 37°C before use.