Дезоксихолатный Гидросульфат лактозы агар культивирование среды (DHL агар)

Application:

DHL agar is used for selective isolation and culture of intestinal pathogenic bacteria, especially Salmonella and Shigella (GB/T4789.28-2003, 4.20, GB/T4789.4-2003 and GB13091-91, SN0170-92 and SN/) T2206.3-2009, SN/T2206.1-2008).

Principle:

Peptone, beef extract powder provides carbon source, nitrogen source, vitamins and minerals; lactose and sucrose are fermentable sugars; sodium deoxycholate and sodium citrate Gram-positive bacteria and most coliforms and deformation Bacillus, but does not affect the growth of Salmonella; sodium thiosulfate and ammonium ferric citrate are used to detect the production of hydrogen sulfide, so that the center of the colony is black; neutral red is the pH indicator, the colony of fermented sugar acid is red, no The colony of the fermented sugar is colorless; the agar is a coagulant of the medium.

Instructions:

1. Weigh 63.3g of DHL agar, add 1 L of distilled water or deionized water, stir and heat to boil until completely dissolved, and then cool to about 50 ° C. In a sterile environment, pour the sterilized plate, and then set aside after solidification.

2. Take a loop of the enrichment solution with the inoculating loop and inoculate the plate on the plate.

3. Place the plate in a constant temperature incubator and incubate at 36 ± 1 °C for 18-24 hours.

4. Observe the results.

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