Высокочувствительный набор реагентов для тестирования на микоплазме

Mycoplasma contamination can cause adverse effects on all aspects of cells, and has become a highly valued issue in cell culture. Therefore, it is very necessary to conduct regular mycoplasma contamination testing. This mycoplasma test kit adopts PCR method to specifically detect mycoplasma contamination in various cultured cell biological materials (such as cell culture, laboratory animal secretions, animal serum, etc.). The mixed primers used in the kit are specific primers designed for the conserved regions of Mycoplasma 16s rRNA sequence. Cell culture fluid can be used directly as a PCR template to specifically amplify Mycoplasma DNA with the matching Mycoplasma PCR Mix (2×), the whole process can be completed in 2 hours. This kit has high detection sensitivity and can detect as few as 20 copies of mycoplasma.

Component:

1. This kit can detect M. Orale, M. Arginini, M. Bovis, M. Fermentans, M. Gallisepticum, M. Hominis, M. Pirum, Ureaplasma SPP, M. Hyorhinis, M. Pneuoniae, A. Laidlawii and others were contaminated by at least 11 species of mycoplasma.
2. Thoroughly defrost and mix all reagents on ice before use, and store them at -20℃ after use.
3. Negative control and positive control must be set for each experiment, and the sample template gradient is recommended for the experimental group.
4. Mask must be worn during operation, strictly in accordance with PCR operation standards, to prevent the introduction of exogenous pollution affecting the experimental results.
5. In order to ensure the reliability and stability of cell experiments, regular detection of mycoplasma contamination is recommended.
6. For your safety and health, please wear a lab coat and disposable gloves to operate.