Сохранить в закладки 1600378853428:
Description:
This product uses a unique lysis buffer system to lyse plant seeds. The lysate can be used as a template without purification. After adding the primers, the PCR Mix of this kit can be used for amplification.
Specifications:
50×20µl rxns,200×20µl rxns,500×20µl rxns,2000×20µl rxns
Kit components:
Plant Seed Direct PCR Kit I | |
Part I | Buffer SP1 |
Buffer SP2 | |
6×DNA Loading Buffer | |
Part II | 2×Seed PCR EasyTM Mix |
Plant Seed Direct PCR Kit II | |
Part I | Buffer SP1 |
Buffer SP2 | |
6×DNA Loading Buffer | |
Part II | 2×Seed PCR EasyTM Mix |
Features&advantages:
-No time-consuming and expensive DNA purification
-Less material
-Simple—the sample can be subjected to PCR reaction or lysis reaction without cutting or grinding
-2 X Mix—reducing sample loading errors and reaction system preparation time
-Fast—template preparation can be completed in 10 minutes, PCR reaction can be completed in 50 minutes
-High throughput—lysis reaction can be completed in 96-well PCR plate
Kit parameters:
Application: Identification of transgenic plants, genotyping, etc.
Sample: Plant seeds or tissues with low polysaccharide and polyphenol content.
Sample size: Single whole seed, 1-100mg plant tissue
Detection range: PCR product ≤1kb
Work Flow:
Electropherogram:
Storage and Shelf life:
-Part I of this kit should be stored at room temperature or 2-8℃.
-Reagents Buffer SP1, Buffer SP2, 6X DNA Loading Buffer, under dry conditions, can be stored for 12 months. If you need to store the kit for a longer time, you can store it at 2-8℃.
-Part II of this kit should be stored at -20℃.
-Reagent 2X Seed PCR Easy Mix, if used frequently, it can also be stored at 4℃ for short-term storage (use up within 10 days).
Foregene Co., Ltd is specialtized in the IVD kits and R&D reagents production and services, which is integrated in R&D, production and marketing. Foregene has the world’s leading Direct PCR platform.The main products include various IVD kits, nucleic acid purification reagents,PCR and Direct PCR reagents,Genotyping kits, etc. The technology and product performance indicators used are all leading the world status,widely applied in hospitals, institutions, medical laboratories, etc.
Foregene Co., Ltd. was founded in April 2011 by well-known scholars who returned from studying at Yale University, famous inventors and senior industry professionals.Foregene’s R&D team consists of 40 people(mainly phD and master), has 2500 m² comprehensive building, 600 m² P2 laboratory, and 1200m² GMP production facility.
With Direct PCR technology, there’s no need for cumbersome nucleic acid purification, the sample is directly added to the system and then on the machine.Now all indicators of Foregene’s Direct PCR technology meet the needs of clinical diagnosis.
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