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PCR and DNA Fragment Purification Kit
For research use only
Kit Content
Content | N1091 | N1092 | N1093 |
Binding Buffer BD | 20 ml | 40 ml | 80 ml |
Wash Buffer PE | 15 ml | 15 mlx2 | 20 mlx3 |
Elution Buffer (10 mM Tris-HCl, pH 8.5) | 2.5 ml | 5 ml | 10 ml |
Spin Columns | 50 each | 100 each | 200 each |
Description
PCR and DNA Fragment Purification Kit is designed for rapid and efficient purification of DNA from PCR and other enzymatic
reaction mixtures. The kit utilizes a proprietary silicabased membrane technology in the form of a convenient spin
column, eliminating the need for tedious resin manipulations or toxic phenolchloroform extractions. The PCR and DNA Fragment Purification Kit effectively removes primers, dNTPs, unincorporated labeled nucleotides, enzymes and salts
from PCR and other reaction mixtures. The kit can be used for purification of DNA fragments from 50 bp to 40 kb with
recoveryates up to 100%. Each purification column has a total binding capacity of up to 30μg of DNA and the entireprocedure takes just 15 min. The purified DNA can be used in common downstream applications such as sequencing restriction digestion, labeling, ligation, cloning, in vitro transcription, blotting or in situ hybridization.
Applications
Fast and efficient purification of DNA fragments ideal for use in all conventional molecular biology
procedures including:
• conventional restriction digestion
• automated fluorescent or radioactive sequencing
• PCR
• in vitro transcription
Feature.
• Fast - procedure takes only 15 min.
• High Efficient – up to 85% recoveries in the range of 50bp-40kb.
• Convenient-spin columns are capped and assembled with collection tubes.
• High purity - OD260/280=1.7-1.9. Purified DNA is ready for downstream application such as PCR, restriction digestion.
Storage
PCR and DNA Fragment Purification Kit can be stored for up to 12 months at room temperature (15-25°C) or at 4°C
for storage periods longer than 12 months. Any precipitate in the buffers can be re-dissolved by incubating at 37°C before use.
Quality Control
The kit is tested in the purification of 50 bp and 1 kb PCR products according to the protocol.
The quality of the purified DNA is evaluated spectrophotometrically, by agarose gel electrophoresis,
digestion with restriction enzymes and automated fluorescent sequencing.
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Dongsheng Biotech PCR Reagents are looking for distributors and providing OEM service
Dongsheng Biotech offers different series of products to help you achieve PCR success. For enzymes, our Taq Polymerase, HS Taq DNA Polymerase, FS Taq DNA Polymerase, Pfu DNA Polymerase and Fusion Pfu DNA Polymerase provide high fidelity, efficient, sensitivity PCR performance. We also have Long Taq DNA Polymerase for long PCR performance.
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